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Mol. Cells 2010; 30(4): 377-382

Published online October 31, 2010

https://doi.org/10.1007/s10059-010-0128-6

© The Korean Society for Molecular and Cellular Biology

The Expression Profile and Function of Satb2 in Zebrafish Embryonic Development

Hyun-Jong Ahn1, Yoojin Park2, Suhyun Kim3, Hae-chul Park3, Su-Kyoung Seo4, Sang-Yeob Yeo4,*, and Dongho Geum2,*

1Department of Microbiology, Kyung Hee University Medical School, Seoul 130-701, Korea, 2Graduate School of Medicine, Brain Korea 21, Korea University, Seoul 136-705, Korea, 3Graduate School of Medicine, Brain Korea 21, Korea University, Ansan 425-707, Korea, 4Department of Biotechnology, Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon 305-719, Korea

Correspondence to : *Correspondence: geumd@korea.ac.kr (DG); yeosy@hanbat.ac.kr (SY)

Received: May 25, 2010; Revised: July 17, 2010; Accepted: July 19, 2010

Abstract

The present study shows the expression profile and function of the homeobox gene, satb2 during zebrafish embryonic development. Satb2 was ubiquitously ex-pressed from the 1 cell stage to the 10-somite stage in zebrafish embryos. Satb2 showed stage-specific expres-sion profiles such as in the pronephric duct at 24 hpf, the branchial arches at 36 hpf, and the ganglion cell layer of the retina and fins at 48 hpf. Additionally, satb2 knock-down embryos were arrested at 50-60% epiboly, and transplantation experiments with satb2 knockdown cells showed migration defects. Interestingly, satb2 knockdown cells also exhibited down-regulation of dynamin II and VAMP4, which are involved in exocytosis and endocytosis, respectively. Furthermore, satb2 knockdown cells have a disorganized actin distribution and an underdeveloped external yolk syncytial layer, both of which are involved in epiboly. These results suggest that satb2 has a functional role in epiboly. This role may potentially be the regulation of endo-exocytic vesicle transport-dependent cell migration and/or the regulation of the development of the yolk syncytial layer.

Keywords epiboly, Satb2, yolk syncytial layer, zebrafish

Article

Research Article

Mol. Cells 2010; 30(4): 377-382

Published online October 31, 2010 https://doi.org/10.1007/s10059-010-0128-6

Copyright © The Korean Society for Molecular and Cellular Biology.

The Expression Profile and Function of Satb2 in Zebrafish Embryonic Development

Hyun-Jong Ahn1, Yoojin Park2, Suhyun Kim3, Hae-chul Park3, Su-Kyoung Seo4, Sang-Yeob Yeo4,*, and Dongho Geum2,*

1Department of Microbiology, Kyung Hee University Medical School, Seoul 130-701, Korea, 2Graduate School of Medicine, Brain Korea 21, Korea University, Seoul 136-705, Korea, 3Graduate School of Medicine, Brain Korea 21, Korea University, Ansan 425-707, Korea, 4Department of Biotechnology, Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon 305-719, Korea

Correspondence to:*Correspondence: geumd@korea.ac.kr (DG); yeosy@hanbat.ac.kr (SY)

Received: May 25, 2010; Revised: July 17, 2010; Accepted: July 19, 2010

Abstract

The present study shows the expression profile and function of the homeobox gene, satb2 during zebrafish embryonic development. Satb2 was ubiquitously ex-pressed from the 1 cell stage to the 10-somite stage in zebrafish embryos. Satb2 showed stage-specific expres-sion profiles such as in the pronephric duct at 24 hpf, the branchial arches at 36 hpf, and the ganglion cell layer of the retina and fins at 48 hpf. Additionally, satb2 knock-down embryos were arrested at 50-60% epiboly, and transplantation experiments with satb2 knockdown cells showed migration defects. Interestingly, satb2 knockdown cells also exhibited down-regulation of dynamin II and VAMP4, which are involved in exocytosis and endocytosis, respectively. Furthermore, satb2 knockdown cells have a disorganized actin distribution and an underdeveloped external yolk syncytial layer, both of which are involved in epiboly. These results suggest that satb2 has a functional role in epiboly. This role may potentially be the regulation of endo-exocytic vesicle transport-dependent cell migration and/or the regulation of the development of the yolk syncytial layer.

Keywords: epiboly, Satb2, yolk syncytial layer, zebrafish

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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