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Mol. Cells 2010; 30(4): 363-368

Published online October 31, 2010

https://doi.org/10.1007/s10059-010-0126-8

© The Korean Society for Molecular and Cellular Biology

The RstB Sensor Acts on the PhoQ Sensor to Control Expression of PhoP-Regulated Genes

Daesil Nam1,3, Eunna Choi1,3, Dae-Hyuk Kweon2, and Dongwoo Shin1,*

1Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea, 2School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Korea, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: dshin@med.skku.ac.kr

Received: May 14, 2010; Revised: July 6, 2010; Accepted: July 8, 2010

Abstract

The PhoP response regulator and PhoQ sensor, which are encoded by the phoPQ operon, constitute the PhoP/PhoQ two-component system. Genome-wide tran-scription ana-lysis revealed that heterologous expression of the RstB protein, a sensor of the RstA/RstB two-component system, leads to enhanced transcription of PhoP-activated genes in wild-type Salmonella. We deter-mined that RstB-induc-tion increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes. This regulatory function of RstB did not require its enzymatic activities, and thus the truncated RstB protein containing only periplasmic and transmembrane regions was able to promote PhoP-activated transcription. The RstB protein appeared to target the PhoQ sensor rather than the PhoP response regulator because RstB-induction failed to enhance transcription of the PhoP-regulated genes in a strain maintaining the normal PhoP function, even without PhoQ.

Keywords crosstalk, PhoP/PhoQ, RstB Sensor, Salmonella, two-component system

Article

Research Article

Mol. Cells 2010; 30(4): 363-368

Published online October 31, 2010 https://doi.org/10.1007/s10059-010-0126-8

Copyright © The Korean Society for Molecular and Cellular Biology.

The RstB Sensor Acts on the PhoQ Sensor to Control Expression of PhoP-Regulated Genes

Daesil Nam1,3, Eunna Choi1,3, Dae-Hyuk Kweon2, and Dongwoo Shin1,*

1Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea, 2School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Korea, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: dshin@med.skku.ac.kr

Received: May 14, 2010; Revised: July 6, 2010; Accepted: July 8, 2010

Abstract

The PhoP response regulator and PhoQ sensor, which are encoded by the phoPQ operon, constitute the PhoP/PhoQ two-component system. Genome-wide tran-scription ana-lysis revealed that heterologous expression of the RstB protein, a sensor of the RstA/RstB two-component system, leads to enhanced transcription of PhoP-activated genes in wild-type Salmonella. We deter-mined that RstB-induc-tion increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes. This regulatory function of RstB did not require its enzymatic activities, and thus the truncated RstB protein containing only periplasmic and transmembrane regions was able to promote PhoP-activated transcription. The RstB protein appeared to target the PhoQ sensor rather than the PhoP response regulator because RstB-induction failed to enhance transcription of the PhoP-regulated genes in a strain maintaining the normal PhoP function, even without PhoQ.

Keywords: crosstalk, PhoP/PhoQ, RstB Sensor, Salmonella, two-component system

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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