Mol. Cells 2010; 30(4): 355-362
Published online September 10, 2010
https://doi.org/10.1007/s10059-010-0134-8
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: khtcat@skku.edu
c-Myc is a cellular onco-protein and a transcriptional activator important for cell growth, cell division, and tumorigenesis. Despite all that is known of its function, the mechanism of how c-Myc contributes to tumorigenesis is unclear. To gain insight into the mechanism through which c-Myc protein exerts its oncogenic activity, we performed large-scale, tandem repeat affinity purification and identified the F box only protein 8 (FBX8), an F-box and Sec7 domain-containing protein, as a novel Myc-binding protein. The c-Myc/FBX8 interaction was mediated by the c-Myc box II (MBII) region. We also confirmed that Myc protein overexpression in 293T cells affected FBX8 cellular trans-location and led to recovery from FBX8-mediated inhibi-tion of ADP-ribosylation factor 6 (ARF6) function during cell invasion. Together, these results suggest that FBX8 is a novel c-Myc binding protein and that c-Myc induces cell invasive activity through the inhibition of FBX8 effects on ARF6 function during cell invasion.
Keywords c-Myc, cell invasion, FBX8, tumorigenesis, ubiquitination
Mol. Cells 2010; 30(4): 355-362
Published online October 31, 2010 https://doi.org/10.1007/s10059-010-0134-8
Copyright © The Korean Society for Molecular and Cellular Biology.
Hyun Jung Cho, Yun Jeong Oh, Junhye Kwon1, Jae Young Kwon, Kyung-Soo Kim2, and Hongtae Kim*
Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea, 1Department of Biological Sciences, Sookmyung Women’s University, Seoul 140-742, Korea, 2Department of Family Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, College of Medicine, Seoul 137-040, Korea
Correspondence to:*Correspondence: khtcat@skku.edu
c-Myc is a cellular onco-protein and a transcriptional activator important for cell growth, cell division, and tumorigenesis. Despite all that is known of its function, the mechanism of how c-Myc contributes to tumorigenesis is unclear. To gain insight into the mechanism through which c-Myc protein exerts its oncogenic activity, we performed large-scale, tandem repeat affinity purification and identified the F box only protein 8 (FBX8), an F-box and Sec7 domain-containing protein, as a novel Myc-binding protein. The c-Myc/FBX8 interaction was mediated by the c-Myc box II (MBII) region. We also confirmed that Myc protein overexpression in 293T cells affected FBX8 cellular trans-location and led to recovery from FBX8-mediated inhibi-tion of ADP-ribosylation factor 6 (ARF6) function during cell invasion. Together, these results suggest that FBX8 is a novel c-Myc binding protein and that c-Myc induces cell invasive activity through the inhibition of FBX8 effects on ARF6 function during cell invasion.
Keywords: c-Myc, cell invasion, FBX8, tumorigenesis, ubiquitination
An Song, Yi Wang, Feng Jiang, Enshi Yan, Junbo Zhou, Jinhai Ye, Hongchuang Zhang, Xu Ding, Gang Li, Yunong Wu, Yang Zheng, and Xiaomeng Song
Mol. Cells 2021; 44(7): 468-480 https://doi.org/10.14348/molcells.2021.2229Yuki Date and Kosei Ito
Mol. Cells 2020; 43(2): 176-181 https://doi.org/10.14348/molcells.2019.0285Michael Lie-a-ling, Renaud Mevel, Rahima Patel, Karen Blyth, Esther Baena, Valerie Kouskoff, and Georges Lacaud
Mol. Cells 2020; 43(2): 126-138 https://doi.org/10.14348/molcells.2019.0301