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Mol. Cells 2010; 30(4): 295-301

Published online September 2, 2010

https://doi.org/10.1007/s10059-010-0118-8

© The Korean Society for Molecular and Cellular Biology

A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences

In-Hyuk Chung1, Hye Sook Yoo2, Jae-Yong Eah2, Hyun-Kyu Yoon1, Jin-Wook Jung1, Seung Yong Hwang1, and Chang-Bae Kim*

Department of Life Science, Sangmyung University, Seoul 110-742, Korea, 1Department of Biochemistry, Hanyang University and GenoCheck Co. Ltd., Ansan 426-791, Korea, 2Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea

Correspondence to : *Correspondence: evodevo@smu.ac.kr

Received: January 11, 2010; Revised: July 15, 2010; Accepted: July 19, 2010

Abstract

DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridiza-tion, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.

Keywords birds, cytochrome c oxidase I, DNA barcoding, Korea, microarray, molecular probes

Article

Research Article

Mol. Cells 2010; 30(4): 295-301

Published online October 31, 2010 https://doi.org/10.1007/s10059-010-0118-8

Copyright © The Korean Society for Molecular and Cellular Biology.

A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences

In-Hyuk Chung1, Hye Sook Yoo2, Jae-Yong Eah2, Hyun-Kyu Yoon1, Jin-Wook Jung1, Seung Yong Hwang1, and Chang-Bae Kim*

Department of Life Science, Sangmyung University, Seoul 110-742, Korea, 1Department of Biochemistry, Hanyang University and GenoCheck Co. Ltd., Ansan 426-791, Korea, 2Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea

Correspondence to:*Correspondence: evodevo@smu.ac.kr

Received: January 11, 2010; Revised: July 15, 2010; Accepted: July 19, 2010

Abstract

DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridiza-tion, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.

Keywords: birds, cytochrome c oxidase I, DNA barcoding, Korea, microarray, molecular probes

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

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