Mol. Cells 2010; 30(4): 295-301
Published online September 2, 2010
https://doi.org/10.1007/s10059-010-0118-8
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: evodevo@smu.ac.kr
DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridiza-tion, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
Keywords birds, cytochrome c oxidase I, DNA barcoding, Korea, microarray, molecular probes
Mol. Cells 2010; 30(4): 295-301
Published online October 31, 2010 https://doi.org/10.1007/s10059-010-0118-8
Copyright © The Korean Society for Molecular and Cellular Biology.
In-Hyuk Chung1, Hye Sook Yoo2, Jae-Yong Eah2, Hyun-Kyu Yoon1, Jin-Wook Jung1, Seung Yong Hwang1, and Chang-Bae Kim*
Department of Life Science, Sangmyung University, Seoul 110-742, Korea, 1Department of Biochemistry, Hanyang University and GenoCheck Co. Ltd., Ansan 426-791, Korea, 2Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea
Correspondence to:*Correspondence: evodevo@smu.ac.kr
DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridiza-tion, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
Keywords: birds, cytochrome c oxidase I, DNA barcoding, Korea, microarray, molecular probes
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