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Mol. Cells 2010; 30(2): 113-119

Published online August 31, 2010

https://doi.org/10.1007/s10059-010-0095-y

© The Korean Society for Molecular and Cellular Biology

Characterization of SHORT-ROOT Function in the Arabidopsis Root Vascular System

Nan-Ie Yu1, Shin Ae Lee1,3, Mi-Hyun Lee1,3, Jung-Ok Heo1, Kwang Suk Chang1,2, and Jun Lim1,2,*

1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea, 2Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: jlim@konkuk.ac.kr

Received: February 1, 2010; Revised: April 19, 2010; Accepted: April 22, 2010

Abstract

Development of the vascular tissues is a dynamic process that integrates extrinsic and intrinsic factors to control vascular tissue formation throughout the plant life cycle. During vascular tissue formation in Arabidopsis roots, radial and longitudinal signals, including nuclear factors and plant hormones, control the developmental processes involved in the specification, differentiation, and maintenance of the correct cell types. SHR, a GRAS transcription factor, has been known to regulate the specification of the stem cell niche and ground tissue identity in the root meristem in a non-cell-autonomous manner. However, the role of SHR in the root vasculature is relatively overlooked, despite localization of its mRNA and protein in the stele. Here, we investigated the role of SHR in the vascular system of the primary root using a reverse genetic approach and detailed phenotypic analysis. A novel, loss-of-function null mutant, shr-6, was isolated in the Columbia background, and vascular patterning was characterized in detail. Our results reveal that shr mutants have developmental defects in both protophloem and protoxylem elements. Our study also suggests that SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals.

Keywords Arabidopsis, GRAS transcription factor, SHORT-ROOT, vascular development

Article

Research Article

Mol. Cells 2010; 30(2): 113-119

Published online August 31, 2010 https://doi.org/10.1007/s10059-010-0095-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Characterization of SHORT-ROOT Function in the Arabidopsis Root Vascular System

Nan-Ie Yu1, Shin Ae Lee1,3, Mi-Hyun Lee1,3, Jung-Ok Heo1, Kwang Suk Chang1,2, and Jun Lim1,2,*

1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea, 2Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: jlim@konkuk.ac.kr

Received: February 1, 2010; Revised: April 19, 2010; Accepted: April 22, 2010

Abstract

Development of the vascular tissues is a dynamic process that integrates extrinsic and intrinsic factors to control vascular tissue formation throughout the plant life cycle. During vascular tissue formation in Arabidopsis roots, radial and longitudinal signals, including nuclear factors and plant hormones, control the developmental processes involved in the specification, differentiation, and maintenance of the correct cell types. SHR, a GRAS transcription factor, has been known to regulate the specification of the stem cell niche and ground tissue identity in the root meristem in a non-cell-autonomous manner. However, the role of SHR in the root vasculature is relatively overlooked, despite localization of its mRNA and protein in the stele. Here, we investigated the role of SHR in the vascular system of the primary root using a reverse genetic approach and detailed phenotypic analysis. A novel, loss-of-function null mutant, shr-6, was isolated in the Columbia background, and vascular patterning was characterized in detail. Our results reveal that shr mutants have developmental defects in both protophloem and protoxylem elements. Our study also suggests that SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals.

Keywords: Arabidopsis, GRAS transcription factor, SHORT-ROOT, vascular development

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

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