TOP

Research Article

Split Viewer

Mol. Cells 2010; 30(2): 113-119

Published online July 23, 2010

https://doi.org/10.1007/s10059-010-0095-y

© The Korean Society for Molecular and Cellular Biology

Characterization of SHORT-ROOT Function in the Arabidopsis Root Vascular System

Nan-Ie Yu1, Shin Ae Lee1,3, Mi-Hyun Lee1,3, Jung-Ok Heo1, Kwang Suk Chang1,2, and Jun Lim1,2,*

1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea, 2Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: jlim@konkuk.ac.kr

Received: February 1, 2010; Revised: April 19, 2010; Accepted: April 22, 2010

Abstract

Development of the vascular tissues is a dynamic process that integrates extrinsic and intrinsic factors to control vascular tissue formation throughout the plant life cycle. During vascular tissue formation in Arabidopsis roots, radial and longitudinal signals, including nuclear factors and plant hormones, control the developmental processes involved in the specification, differentiation, and maintenance of the correct cell types. SHR, a GRAS transcription factor, has been known to regulate the specification of the stem cell niche and ground tissue identity in the root meristem in a non-cell-autonomous manner. However, the role of SHR in the root vasculature is relatively overlooked, despite localization of its mRNA and protein in the stele. Here, we investigated the role of SHR in the vascular system of the primary root using a reverse genetic approach and detailed phenotypic analysis. A novel, loss-of-function null mutant, shr-6, was isolated in the Columbia background, and vascular patterning was characterized in detail. Our results reveal that shr mutants have developmental defects in both protophloem and protoxylem elements. Our study also suggests that SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals.

Keywords Arabidopsis, GRAS transcription factor, SHORT-ROOT, vascular development

Article

Research Article

Mol. Cells 2010; 30(2): 113-119

Published online August 31, 2010 https://doi.org/10.1007/s10059-010-0095-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Characterization of SHORT-ROOT Function in the Arabidopsis Root Vascular System

Nan-Ie Yu1, Shin Ae Lee1,3, Mi-Hyun Lee1,3, Jung-Ok Heo1, Kwang Suk Chang1,2, and Jun Lim1,2,*

1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea, 2Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: jlim@konkuk.ac.kr

Received: February 1, 2010; Revised: April 19, 2010; Accepted: April 22, 2010

Abstract

Development of the vascular tissues is a dynamic process that integrates extrinsic and intrinsic factors to control vascular tissue formation throughout the plant life cycle. During vascular tissue formation in Arabidopsis roots, radial and longitudinal signals, including nuclear factors and plant hormones, control the developmental processes involved in the specification, differentiation, and maintenance of the correct cell types. SHR, a GRAS transcription factor, has been known to regulate the specification of the stem cell niche and ground tissue identity in the root meristem in a non-cell-autonomous manner. However, the role of SHR in the root vasculature is relatively overlooked, despite localization of its mRNA and protein in the stele. Here, we investigated the role of SHR in the vascular system of the primary root using a reverse genetic approach and detailed phenotypic analysis. A novel, loss-of-function null mutant, shr-6, was isolated in the Columbia background, and vascular patterning was characterized in detail. Our results reveal that shr mutants have developmental defects in both protophloem and protoxylem elements. Our study also suggests that SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals.

Keywords: Arabidopsis, GRAS transcription factor, SHORT-ROOT, vascular development

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

Supplementary File

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download