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Mol. Cells 2010; 30(1): 59-64

Published online July 31, 2010

https://doi.org/10.1007/s10059-010-0088-x

© The Korean Society for Molecular and Cellular Biology

C1qTNF-Related Protein-6 Increases the Expression of Interleukin-10 in Macrophages

Mi-Jin Kim1, Wan Lee1, Eun-Ju Park, and Seung-Yoon Park*

Department of Biochemistry, School of Medicine, Dongguk University, Kyungju 780-714, Korea, 1These authors contributed equally to this work.

Correspondence to : *Correspondence: psyoon@dongguk.ac.kr

Received: February 9, 2010; Revised: April 9, 2010; Accepted: April 15, 2010

Abstract

C1qTNF-Related proteins (CTRPs), a new highly con-served family of adiponectin paralogs, were recently identified as being involved in diverse processes including metabolism, host defense, apoptosis, cell differentiation, and organogenesis. However, the functional role of CTRP6 remains poorly identified. Here we provide evidence that CTRP6 induces the expression of interleukin-10 (IL-10) in macrophages. Conditioned medium from CTRP6-expres-sing HEK293 cells increased IL-10 expression in Raw264.7 cells. The globular domain of CTRP6 (gCTRP6) also dose-dependently increased both IL-10 mRNA and protein expression levels, with transcript levels increasing within 2 h. Furthermore, the globular domain of CTRP6 rapidly induced phosphorylation of ERK1/2 in Raw264.7 cells. Treatment with U0126, a selective inhibitor, abolished CTRP6-stimulated IL-10 induction. Taken together, there results demonstrate that CTRP6 induces expression of IL-10 via ERK1/2 activation. Considering that IL-10 is a potent anti-inflammatory cytokine that modulates inflammatory signaling pathways, CTRP6 may be a novel target for pharmacological drugs in inflammatory diseases.

Keywords C1qTNF6, CTRP6, ERK1/2, interleukin-10, macrophages

Article

Research Article

Mol. Cells 2010; 30(1): 59-64

Published online July 31, 2010 https://doi.org/10.1007/s10059-010-0088-x

Copyright © The Korean Society for Molecular and Cellular Biology.

C1qTNF-Related Protein-6 Increases the Expression of Interleukin-10 in Macrophages

Mi-Jin Kim1, Wan Lee1, Eun-Ju Park, and Seung-Yoon Park*

Department of Biochemistry, School of Medicine, Dongguk University, Kyungju 780-714, Korea, 1These authors contributed equally to this work.

Correspondence to:*Correspondence: psyoon@dongguk.ac.kr

Received: February 9, 2010; Revised: April 9, 2010; Accepted: April 15, 2010

Abstract

C1qTNF-Related proteins (CTRPs), a new highly con-served family of adiponectin paralogs, were recently identified as being involved in diverse processes including metabolism, host defense, apoptosis, cell differentiation, and organogenesis. However, the functional role of CTRP6 remains poorly identified. Here we provide evidence that CTRP6 induces the expression of interleukin-10 (IL-10) in macrophages. Conditioned medium from CTRP6-expres-sing HEK293 cells increased IL-10 expression in Raw264.7 cells. The globular domain of CTRP6 (gCTRP6) also dose-dependently increased both IL-10 mRNA and protein expression levels, with transcript levels increasing within 2 h. Furthermore, the globular domain of CTRP6 rapidly induced phosphorylation of ERK1/2 in Raw264.7 cells. Treatment with U0126, a selective inhibitor, abolished CTRP6-stimulated IL-10 induction. Taken together, there results demonstrate that CTRP6 induces expression of IL-10 via ERK1/2 activation. Considering that IL-10 is a potent anti-inflammatory cytokine that modulates inflammatory signaling pathways, CTRP6 may be a novel target for pharmacological drugs in inflammatory diseases.

Keywords: C1qTNF6, CTRP6, ERK1/2, interleukin-10, macrophages

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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