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Mol. Cells 2010; 30(1): 37-49

Published online July 31, 2010

https://doi.org/10.1007/s10059-010-0086-z

© The Korean Society for Molecular and Cellular Biology

Inhibition of Biphasic Ethylene Production Enhances Tolerance to Abiotic Stress by Reducing the Accumulation of Reactive Oxygen Species Nicotiana tabacum

Soo Jin Wi1, Su Jin Jang, and Ky Young Park*

Department of Biology, Sunchon National University, Sunchon 540-742, Korea, 1Korea Basic Science Institute, Sunchon Branch, Sunchon National University, Sunchon 540-742, Korea

Correspondence to : *Correspondence: plpm@sunchon.ac.kr

Received: January 6, 2010; Revised: March 30, 2010; Accepted: March 31, 2010

Abstract

Reactive oxygen species (ROS), such as H2O2, are impor-tant plant cell signaling molecules involved in responses to biotic and abiotic stresses and in developmental and physiological processes. Despite the well-known physio-logical functions of ethylene production and stress signaling via ROS during stresses, whether ethylene acts alone or in conjunction with ROS has not yet been fully elucidated. Therefore, we investigated the relationship between ethylene production and ROS accumulation during the response to abiotic stress. We used three independent transgenic tobacco lines, CAS-AS-2, -3 and -4, in which an antisense transcript of the senescence-related ACC synthase (ACS) gene from carnation flower (CARACC, GenBank accession No. M66619) was expressed heterologously. Biphasic ethylene biosynthesis was reduced significantly in these transgenic plants, with or without H2O2 treatment. These plants exhibited significantly reduced H2O2-induced gene-specific expression of ACS members, which were regulated in a time-dependent manner. The higher levels of NtACS1 expression in wild-type plants led to a second peak in ethylene production, which resulted in a more severe level of necrosis and cell death, as determined by trypan blue staining. In the transgenic lines, upregulated transcription of CAB, POR1 and RbcS resulted in increased photosynthetic performance following salt stress. This stress tolerance of H2O2-treated transgenic plants resulted from reduced ethylene biosynthesis, which decreased ROS accumulation via increased gene expression and activity of ROS-detoxifying enzymes, including MnSOD, CuZnSOD, and catalase. Therefore, it is suggested that ethylene plays a potentially critical role as an amplifier for ROS accumulation, implying a synergistic effect between biosynthesis of ROS and ethylene.

Keywords abiotic stress, biphasic, ethylene, reactive oxygen species, tolerance

Article

Research Article

Mol. Cells 2010; 30(1): 37-49

Published online July 31, 2010 https://doi.org/10.1007/s10059-010-0086-z

Copyright © The Korean Society for Molecular and Cellular Biology.

Inhibition of Biphasic Ethylene Production Enhances Tolerance to Abiotic Stress by Reducing the Accumulation of Reactive Oxygen Species Nicotiana tabacum

Soo Jin Wi1, Su Jin Jang, and Ky Young Park*

Department of Biology, Sunchon National University, Sunchon 540-742, Korea, 1Korea Basic Science Institute, Sunchon Branch, Sunchon National University, Sunchon 540-742, Korea

Correspondence to:*Correspondence: plpm@sunchon.ac.kr

Received: January 6, 2010; Revised: March 30, 2010; Accepted: March 31, 2010

Abstract

Reactive oxygen species (ROS), such as H2O2, are impor-tant plant cell signaling molecules involved in responses to biotic and abiotic stresses and in developmental and physiological processes. Despite the well-known physio-logical functions of ethylene production and stress signaling via ROS during stresses, whether ethylene acts alone or in conjunction with ROS has not yet been fully elucidated. Therefore, we investigated the relationship between ethylene production and ROS accumulation during the response to abiotic stress. We used three independent transgenic tobacco lines, CAS-AS-2, -3 and -4, in which an antisense transcript of the senescence-related ACC synthase (ACS) gene from carnation flower (CARACC, GenBank accession No. M66619) was expressed heterologously. Biphasic ethylene biosynthesis was reduced significantly in these transgenic plants, with or without H2O2 treatment. These plants exhibited significantly reduced H2O2-induced gene-specific expression of ACS members, which were regulated in a time-dependent manner. The higher levels of NtACS1 expression in wild-type plants led to a second peak in ethylene production, which resulted in a more severe level of necrosis and cell death, as determined by trypan blue staining. In the transgenic lines, upregulated transcription of CAB, POR1 and RbcS resulted in increased photosynthetic performance following salt stress. This stress tolerance of H2O2-treated transgenic plants resulted from reduced ethylene biosynthesis, which decreased ROS accumulation via increased gene expression and activity of ROS-detoxifying enzymes, including MnSOD, CuZnSOD, and catalase. Therefore, it is suggested that ethylene plays a potentially critical role as an amplifier for ROS accumulation, implying a synergistic effect between biosynthesis of ROS and ethylene.

Keywords: abiotic stress, biphasic, ethylene, reactive oxygen species, tolerance

Mol. Cells
Jun 30, 2022 Vol.45 No.6, pp. 353~434
COVER PICTURE
ERα is modified by UFM1 and this modification (ufmylation) plays a crucial role in promoting the stability of ERα and breast cancer development. However, when ERα is deufmylated and then ubiquitinated, it disappears by proteasome-mediated degradation (Yoo et al., pp. 425-434).

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