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Mol. Cells 2010; 30(1): 19-27

Published online July 31, 2010

https://doi.org/10.1007/s10059-010-0084-1

© The Korean Society for Molecular and Cellular Biology

OsCIPK31, a CBL-Interacting Protein Kinase Is Involved in Germination and Seedling Growth under Abiotic Stress Conditions in Rice Plants

Hai-long Piao1,7, Yuan-hu Xuan1,7, Su Hyun Park1,7, Byoung Il Je1, Soon Ju Park1, Sung Han Park1,5, Chul Min Kim1,6, Jin Huang1, Guo Kui Wang1, Min Jung Kim1, Sang Mo Kang2, In-Jung Lee2,Taek-Ryoun Kwon3, Yong Hwan Kim3, Un-sang Yeo4, Gihwan Yi4, DaeYoung Son1, and Chang-deok Han1,*

1Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea, 2Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea, 3National Academy of Agriculture Science, Rural Development Administration, Suwon 441-857, Korea, 4National Institute of Crop Science, Rural Development Administration, Milyang 627-803, Korea, 5Present address: Rice Functional Genomics, National institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea, 6Present address: Department of Cell and Development Biology, John Innes Center, UK, 7These authors contributed equally to this work.

Correspondence to : *Correspondence: cdhan@gsnu.ac.kr

Received: December 4, 2010; Revised: March 26, 2010; Accepted: April 7, 2010

Abstract

Calcineurin B-like protein-interacting protein kinases (CIPKs) are a group of typical Ser/Thr protein kinases that mediate calcium signals. Extensive studies using Arabidopsis plants have demonstrated that many calcium signatures that activate CIPKs originate from abiotic stresses. However, there are few reports on the functional demonstration of CIPKs in other plants, es-pecially in grasses. In this study, we used a loss-of-function mutation to characterize the function of the rice CIPK gene OsCIPK31. Exposure to high concentrations of NaCl or mannitol effected a rapid and transient enhancement of OsCIPK31 expression. These findings were observed only in the light. However, longer expo-sure to most stresses resulted in down-regulation of OsCIPK31 expression in both the presence and absence of light. To determine the physiological roles of OsCIPK31 in rice plants, the sensitivity of oscipk31::Ds, which is a transposon Ds insertion mutant, to abiotic stresses was examined during germination and seedling stages. oscipk31::Ds mutants exhibited hypersensitive phenotypes to ABA, salt, mannitol, and glucose. Com-pared with wild-type rice plants, mutants exhibited re-tarded germination and slow seedling growth. In addi-tion, oscipk31::Ds seedlings exhibited enhanced ex-pression of several stress-responsive genes after ex-posure to these abiotic stresses. However, the expression of ABA metabolic genes and the endogenous levels of ABA were not altered significantly in the oscipk31::Ds mutant. This study demonstrated that rice plants use OsCIPK31 to modulate responses to abiotic stresses during the seed germination and seedling stages and to modulate the expression of stress-responsive genes.

Keywords abiotic stresses, Calcineurin B-like protein-interacting protein kinases (CIPKs), Ds insertion mutants, hypersensitive, rice

Article

Research Article

Mol. Cells 2010; 30(1): 19-27

Published online July 31, 2010 https://doi.org/10.1007/s10059-010-0084-1

Copyright © The Korean Society for Molecular and Cellular Biology.

OsCIPK31, a CBL-Interacting Protein Kinase Is Involved in Germination and Seedling Growth under Abiotic Stress Conditions in Rice Plants

Hai-long Piao1,7, Yuan-hu Xuan1,7, Su Hyun Park1,7, Byoung Il Je1, Soon Ju Park1, Sung Han Park1,5, Chul Min Kim1,6, Jin Huang1, Guo Kui Wang1, Min Jung Kim1, Sang Mo Kang2, In-Jung Lee2,Taek-Ryoun Kwon3, Yong Hwan Kim3, Un-sang Yeo4, Gihwan Yi4, DaeYoung Son1, and Chang-deok Han1,*

1Division of Applied Life Science (Brain Korea 21 Program), Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea, 2Division of Plant Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea, 3National Academy of Agriculture Science, Rural Development Administration, Suwon 441-857, Korea, 4National Institute of Crop Science, Rural Development Administration, Milyang 627-803, Korea, 5Present address: Rice Functional Genomics, National institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea, 6Present address: Department of Cell and Development Biology, John Innes Center, UK, 7These authors contributed equally to this work.

Correspondence to:*Correspondence: cdhan@gsnu.ac.kr

Received: December 4, 2010; Revised: March 26, 2010; Accepted: April 7, 2010

Abstract

Calcineurin B-like protein-interacting protein kinases (CIPKs) are a group of typical Ser/Thr protein kinases that mediate calcium signals. Extensive studies using Arabidopsis plants have demonstrated that many calcium signatures that activate CIPKs originate from abiotic stresses. However, there are few reports on the functional demonstration of CIPKs in other plants, es-pecially in grasses. In this study, we used a loss-of-function mutation to characterize the function of the rice CIPK gene OsCIPK31. Exposure to high concentrations of NaCl or mannitol effected a rapid and transient enhancement of OsCIPK31 expression. These findings were observed only in the light. However, longer expo-sure to most stresses resulted in down-regulation of OsCIPK31 expression in both the presence and absence of light. To determine the physiological roles of OsCIPK31 in rice plants, the sensitivity of oscipk31::Ds, which is a transposon Ds insertion mutant, to abiotic stresses was examined during germination and seedling stages. oscipk31::Ds mutants exhibited hypersensitive phenotypes to ABA, salt, mannitol, and glucose. Com-pared with wild-type rice plants, mutants exhibited re-tarded germination and slow seedling growth. In addi-tion, oscipk31::Ds seedlings exhibited enhanced ex-pression of several stress-responsive genes after ex-posure to these abiotic stresses. However, the expression of ABA metabolic genes and the endogenous levels of ABA were not altered significantly in the oscipk31::Ds mutant. This study demonstrated that rice plants use OsCIPK31 to modulate responses to abiotic stresses during the seed germination and seedling stages and to modulate the expression of stress-responsive genes.

Keywords: abiotic stresses, Calcineurin B-like protein-interacting protein kinases (CIPKs), Ds insertion mutants, hypersensitive, rice

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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