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Mol. Cells 2010; 29(6): 559-566

Published online June 30, 2010

https://doi.org/10.1007/s10059-010-0070-7

© The Korean Society for Molecular and Cellular Biology

AtNEK6 Interacts with ARIA and Is Involved in ABA Response during Seed Germination

Sun-ji Lee, Dong-Im Cho, Jung-youn Kang, Myung-Duck Kim, and Soo Young Kim*

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Correspondence to : *Correspondence: sooykim@chonnam.ac.kr

Received: November 3, 2010; Revised: February 10, 2010; Accepted: February 18, 2010

Abstract

ARIA is an ARM repeat protein that is a positive regulator of ABA response. To identify ARIA-interacting proteins, we conducted yeast two-hybrid screening. One of the positive clones obtained from the screen encoded a protein kinase, AtNEK6, which belongs to the NIMA (Never In Mitosis, gene A)-related kinase family. We analyzed AtNEK6 overexpression (OX) and knockout (KO) lines to investigate its in vivo function. The AtNEK6 OX lines grew slowly, where-as the KO line germinated and grew faster than wild type plants. AtNEK6 also affected ABA and stress responses. During seed germination, AtNEK6 OX lines were hypersensitive to ABA and high osmolarity, whereas its KO line was partially insensitive to ABA and high osmolarity. Pre-viously, AtNEK6 was shown to be involved in epidermal cell morphogenesis. Our results indicate that AtNEK6 is also involved in plant growth regulation and responses to ABA and high osmolarity during the seed germination stage.

Keywords abiotic stress, abscisic acid (ABA), Arabidopsis, protein kinase, two-hybrid screen

Article

Research Article

Mol. Cells 2010; 29(6): 559-566

Published online June 30, 2010 https://doi.org/10.1007/s10059-010-0070-7

Copyright © The Korean Society for Molecular and Cellular Biology.

AtNEK6 Interacts with ARIA and Is Involved in ABA Response during Seed Germination

Sun-ji Lee, Dong-Im Cho, Jung-youn Kang, Myung-Duck Kim, and Soo Young Kim*

Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea

Correspondence to:*Correspondence: sooykim@chonnam.ac.kr

Received: November 3, 2010; Revised: February 10, 2010; Accepted: February 18, 2010

Abstract

ARIA is an ARM repeat protein that is a positive regulator of ABA response. To identify ARIA-interacting proteins, we conducted yeast two-hybrid screening. One of the positive clones obtained from the screen encoded a protein kinase, AtNEK6, which belongs to the NIMA (Never In Mitosis, gene A)-related kinase family. We analyzed AtNEK6 overexpression (OX) and knockout (KO) lines to investigate its in vivo function. The AtNEK6 OX lines grew slowly, where-as the KO line germinated and grew faster than wild type plants. AtNEK6 also affected ABA and stress responses. During seed germination, AtNEK6 OX lines were hypersensitive to ABA and high osmolarity, whereas its KO line was partially insensitive to ABA and high osmolarity. Pre-viously, AtNEK6 was shown to be involved in epidermal cell morphogenesis. Our results indicate that AtNEK6 is also involved in plant growth regulation and responses to ABA and high osmolarity during the seed germination stage.

Keywords: abiotic stress, abscisic acid (ABA), Arabidopsis, protein kinase, two-hybrid screen

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

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