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Mol. Cells 2010; 29(6): 551-558

Published online May 20, 2010

https://doi.org/10.1007/s10059-010-0069-0

© The Korean Society for Molecular and Cellular Biology

Zinc Deficiency-Inducible OsZIP8 Encodes a Plasma Membrane-Localized Zinc Transporter in Rice

Sichul Lee1,2, Sun A Kim2, Joohyun Lee2, Mary Lou Guerinot2, and Gynheung An1,3,*

1Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea, 2Department of Biological Sciences, Dartmouth College, Hanover, USA, 3Department of Plant Molecular Systems Biotechnology and Crop Biotech Center, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: genean@postech.ac.kr

Received: October 19, 2010; Revised: February 11, 2010; Accepted: February 11, 2010

Abstract

Zinc is an essential micronutrient for several physiological and biochemical processes. To investigate its transport in rice, we characterized OsZIP8, a rice ZIP (Zrt, Irt-like Protein) gene that is strongly up-regulated in shoots and roots under Zn deficiency. OsZIP8 could complement the growth defect of Zn-uptake yeast mutant. The OsZIP8-GFP fusion proteins were localized to the plasma membrane, suggesting that OsZIP8 is a plasma membrane zinc transporter in rice. Activation and overexpression of this gene disturbed the zinc distribution in rice plants, resulting in lower levels in shoots and mature seeds, but an increase in the roots. Field-grown transgenic plants were shorter than the WT. Under treatment with excess zinc, transgenics contained less zinc in their shoots but accumulated more in the roots. Altogether, these results demonstrate that OsZIP8 is a zinc transporter that functions in Zn uptake and distribution. Furthermore, zinc homeostasis is important to the proper growth and development of rice.

Keywords activation tagging, OsZIP8, overexpression, rice, zinc transporter

Article

Research Article

Mol. Cells 2010; 29(6): 551-558

Published online June 30, 2010 https://doi.org/10.1007/s10059-010-0069-0

Copyright © The Korean Society for Molecular and Cellular Biology.

Zinc Deficiency-Inducible OsZIP8 Encodes a Plasma Membrane-Localized Zinc Transporter in Rice

Sichul Lee1,2, Sun A Kim2, Joohyun Lee2, Mary Lou Guerinot2, and Gynheung An1,3,*

1Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea, 2Department of Biological Sciences, Dartmouth College, Hanover, USA, 3Department of Plant Molecular Systems Biotechnology and Crop Biotech Center, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: genean@postech.ac.kr

Received: October 19, 2010; Revised: February 11, 2010; Accepted: February 11, 2010

Abstract

Zinc is an essential micronutrient for several physiological and biochemical processes. To investigate its transport in rice, we characterized OsZIP8, a rice ZIP (Zrt, Irt-like Protein) gene that is strongly up-regulated in shoots and roots under Zn deficiency. OsZIP8 could complement the growth defect of Zn-uptake yeast mutant. The OsZIP8-GFP fusion proteins were localized to the plasma membrane, suggesting that OsZIP8 is a plasma membrane zinc transporter in rice. Activation and overexpression of this gene disturbed the zinc distribution in rice plants, resulting in lower levels in shoots and mature seeds, but an increase in the roots. Field-grown transgenic plants were shorter than the WT. Under treatment with excess zinc, transgenics contained less zinc in their shoots but accumulated more in the roots. Altogether, these results demonstrate that OsZIP8 is a zinc transporter that functions in Zn uptake and distribution. Furthermore, zinc homeostasis is important to the proper growth and development of rice.

Keywords: activation tagging, OsZIP8, overexpression, rice, zinc transporter

Mol. Cells
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