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Mol. Cells 2010; 29(5): 527-531

Published online April 12, 2010

https://doi.org/10.1007/s10059-010-0065-4

© The Korean Society for Molecular and Cellular Biology

Heat Shock Factor 1 Is a Transcription Factor of Fas Gene

Shunmei E.1, Yuanbo Zhao, Yunhong Huang, Kun Lai, Cha Chen1, Jianming Zeng1, and Jiangying Zou*

Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China, 1The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510120, China

Correspondence to : *Correspondence: zou_jiang_ying@hotmail.com

Received: December 30, 2010; Revised: January 28, 2010; Accepted: January 29, 2010

Abstract

In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.

Keywords Apoptosis, Fas, heat shock factor 1 (HSF1)

Article

Research Article

Mol. Cells 2010; 29(5): 527-531

Published online May 31, 2010 https://doi.org/10.1007/s10059-010-0065-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Heat Shock Factor 1 Is a Transcription Factor of Fas Gene

Shunmei E.1, Yuanbo Zhao, Yunhong Huang, Kun Lai, Cha Chen1, Jianming Zeng1, and Jiangying Zou*

Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China, 1The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510120, China

Correspondence to:*Correspondence: zou_jiang_ying@hotmail.com

Received: December 30, 2010; Revised: January 28, 2010; Accepted: January 29, 2010

Abstract

In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.

Keywords: Apoptosis, Fas, heat shock factor 1 (HSF1)

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

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