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Mol. Cells 2010; 29(5): 527-531
Published online April 12, 2010
https://doi.org/10.1007/s10059-010-0065-4
© The Korean Society for Molecular and Cellular Biology
Heat Shock Factor 1 Is a Transcription Factor of Fas Gene
Correspondence to : *Correspondence: zou_jiang_ying@hotmail.com
In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.
Keywords Apoptosis, Fas, heat shock factor 1 (HSF1)
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Research Article
Mol. Cells 2010; 29(5): 527-531
Published online May 31, 2010 https://doi.org/10.1007/s10059-010-0065-4
Copyright © The Korean Society for Molecular and Cellular Biology.
Heat Shock Factor 1 Is a Transcription Factor of Fas Gene
Shunmei E.1, Yuanbo Zhao, Yunhong Huang, Kun Lai, Cha Chen1, Jianming Zeng1, and Jiangying Zou*
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China, 1The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510120, China
Correspondence to:*Correspondence: zou_jiang_ying@hotmail.com
Abstract
In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.
Keywords: Apoptosis, Fas, heat shock factor 1 (HSF1)
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