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Mol. Cells 2010; 29(5): 527-531

Published online April 12, 2010

https://doi.org/10.1007/s10059-010-0065-4

© The Korean Society for Molecular and Cellular Biology

Heat Shock Factor 1 Is a Transcription Factor of Fas Gene

Shunmei E.1, Yuanbo Zhao, Yunhong Huang, Kun Lai, Cha Chen1, Jianming Zeng1, and Jiangying Zou*

Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China, 1The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510120, China

Correspondence to : *Correspondence: zou_jiang_ying@hotmail.com

Received: December 30, 2010; Revised: January 28, 2010; Accepted: January 29, 2010

Abstract

In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.

Keywords Apoptosis, Fas, heat shock factor 1 (HSF1)

Article

Research Article

Mol. Cells 2010; 29(5): 527-531

Published online May 31, 2010 https://doi.org/10.1007/s10059-010-0065-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Heat Shock Factor 1 Is a Transcription Factor of Fas Gene

Shunmei E.1, Yuanbo Zhao, Yunhong Huang, Kun Lai, Cha Chen1, Jianming Zeng1, and Jiangying Zou*

Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China, 1The Tradition Chinese Hospital of Guangdong Province, Guangzhou 510120, China

Correspondence to:*Correspondence: zou_jiang_ying@hotmail.com

Received: December 30, 2010; Revised: January 28, 2010; Accepted: January 29, 2010

Abstract

In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5´-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the ab-sence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results sug-gested that HSF1 is an important transcription factor of Fas gene.

Keywords: Apoptosis, Fas, heat shock factor 1 (HSF1)

Mol. Cells
Jun 30, 2023 Vol.46 No.6, pp. 329~398
COVER PICTURE
The cellular proteostasis network is adaptively modulated upon cellular stress, thereby protecting cells from proteostasis collapse. Heat shock induces the translocation of misfolded proteins and the chaperone protein HSP70 into nucleolus, where nuclear protein quality control primarily occurs. Nuclear RNA export factor 1 (green), nucleolar protein fibrillarin (red), and nuclei (blue) were visualized in NIH3T3 cells under basal (left) and heat shock (right) conditions (Park et al., pp. 374-386).

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