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Mol. Cells 2010; 29(4): 373-381

Published online March 4, 2010

https://doi.org/10.1007/s10059-010-0046-7

© The Korean Society for Molecular and Cellular Biology

Phosphorylated Extracellular Signal-Regulated Kinase 1/2 Immunoreactivity and Its Protein Levels in the Gerbil Hippocampus during Normal Aging

Choong Hyun Lee1,7, Ki-Yeon Yoo1,7, Ok Kyu Park1, Jung Hoon Choi1, Il-Jun Kang2,*, Eunjoo Bae3,
Sung Koo Kim4, In Koo Hwang5, and Moo-Ho Won1,6,*

1Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Korea, 2Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea, 3Department of Pediatrics, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-703, Korea, 4Department of Pediatrics, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 150-950, Korea, 5Department of Anatomy and Cell Biology, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea, 6MRC Research Institute, Hallym University, Chuncheon 200-702, Korea, 7These authors contributed equally to this work.

Correspondence to : *Correspondence: mhwon@hallym.ac.kr (MW); ijkang@hallym.ac.kr (IK)

Received: October 19, 2009; Revised: December 14, 2009; Accepted: December 17, 2009

Abstract

Phosphorylated extracellular signal-regulated kinase (pERK) mediates neuronal synaptic plasticity, long-term potentiation, and learning and memory in the hippocam-pus. In this study, we examined pERK1/2 immunoreac-tivity and its protein level in the gerbil hippocampus at various ages. In the postnatal month 1 (PM 1) group, very weak pERK1/2 immunoreactivity was detected in the hippocampus. In the CA1 region, pERK1/2 immuno-reactivity was considerably increased in the stratum pyramidale in the PM 6 group. Thereafter, pERK1/2 im-munoreactivity was decreased. In the CA2/3 region, pERK1/2 immunoreactivity increased in an age-dependent manner until PM 12. Thereafter, numbers of pERK1/2-immunoreactive neurons were decreased. However, in the mossy fiber zone, pERK1/2 immunostaining became stronger with age. In the dentate gyrus, a few pERK1/2-immunoreactive cells were observed until PM 12. In the PM 18 and 24 groups, numbers of pERK1/2-immunoreactive cells were increased, especially in the polymorphic layer. In Western blot analysis, pERK1/2 level in the gerbil hippocampus was increased with age. These results indicate that total pERK1/2 levels are increased in the hippocampus with age. However pERK1/2 immunoreactivity in subregions of the gerbil hippocampus was changed with different pattern during normal aging.

Keywords aging, gerbil, hippocampus, mossy fiber zone, phosphorylated extracellular signal-regulated kinases 1/2

Article

Research Article

Mol. Cells 2010; 29(4): 373-381

Published online March 4, 2010 https://doi.org/10.1007/s10059-010-0046-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Phosphorylated Extracellular Signal-Regulated Kinase 1/2 Immunoreactivity and Its Protein Levels in the Gerbil Hippocampus during Normal Aging

Choong Hyun Lee1,7, Ki-Yeon Yoo1,7, Ok Kyu Park1, Jung Hoon Choi1, Il-Jun Kang2,*, Eunjoo Bae3,
Sung Koo Kim4, In Koo Hwang5, and Moo-Ho Won1,6,*

1Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Korea, 2Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Korea, 3Department of Pediatrics, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-703, Korea, 4Department of Pediatrics, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 150-950, Korea, 5Department of Anatomy and Cell Biology, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea, 6MRC Research Institute, Hallym University, Chuncheon 200-702, Korea, 7These authors contributed equally to this work.

Correspondence to:*Correspondence: mhwon@hallym.ac.kr (MW); ijkang@hallym.ac.kr (IK)

Received: October 19, 2009; Revised: December 14, 2009; Accepted: December 17, 2009

Abstract

Phosphorylated extracellular signal-regulated kinase (pERK) mediates neuronal synaptic plasticity, long-term potentiation, and learning and memory in the hippocam-pus. In this study, we examined pERK1/2 immunoreac-tivity and its protein level in the gerbil hippocampus at various ages. In the postnatal month 1 (PM 1) group, very weak pERK1/2 immunoreactivity was detected in the hippocampus. In the CA1 region, pERK1/2 immuno-reactivity was considerably increased in the stratum pyramidale in the PM 6 group. Thereafter, pERK1/2 im-munoreactivity was decreased. In the CA2/3 region, pERK1/2 immunoreactivity increased in an age-dependent manner until PM 12. Thereafter, numbers of pERK1/2-immunoreactive neurons were decreased. However, in the mossy fiber zone, pERK1/2 immunostaining became stronger with age. In the dentate gyrus, a few pERK1/2-immunoreactive cells were observed until PM 12. In the PM 18 and 24 groups, numbers of pERK1/2-immunoreactive cells were increased, especially in the polymorphic layer. In Western blot analysis, pERK1/2 level in the gerbil hippocampus was increased with age. These results indicate that total pERK1/2 levels are increased in the hippocampus with age. However pERK1/2 immunoreactivity in subregions of the gerbil hippocampus was changed with different pattern during normal aging.

Keywords: aging, gerbil, hippocampus, mossy fiber zone, phosphorylated extracellular signal-regulated kinases 1/2

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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