Top

Research Article

Split Viewer

Mol. Cells 2010; 29(4): 363-371

Published online March 4, 2010

https://doi.org/10.1007/s10059-010-0045-8

© The Korean Society for Molecular and Cellular Biology

An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress

Baolei Jia1, Sangmin Lee1, Bang P. Pham1, Yoon Seung Cho1, Jae-Kyung Yang2, Hee-Seop Byeon2,
Jong Cheol Kim3, and Gang-Won Cheong1,4,*

1Division of Applied Life Sciences (Brain Korea 21 Program), Gyeongsang National University, Jinju 660-701, Korea, 2Division of Environmental Forest Science, Gyeongsang National University, Jinju 660-701, Korea, 3Institute of Hadong Green Tea, Hadong 667-882, Korea, 4Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea

Correspondence to : *Correspondence: gwcheong@gnu.ac.kr

Received: December 4, 2009; Revised: December 18, 2010; Accepted: December 23, 2009

Abstract

NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to H2O2 or four-electron reduction of oxygen to H2O. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to β-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of β-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce H2O2. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treat-ment with H2O2. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.

Keywords cell death, dual function, NADH oxidase, protein aggregation, thermophilic archaeon

Article

Research Article

Mol. Cells 2010; 29(4): 363-371

Published online March 4, 2010 https://doi.org/10.1007/s10059-010-0045-8

Copyright © The Korean Society for Molecular and Cellular Biology.

An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress

Baolei Jia1, Sangmin Lee1, Bang P. Pham1, Yoon Seung Cho1, Jae-Kyung Yang2, Hee-Seop Byeon2,
Jong Cheol Kim3, and Gang-Won Cheong1,4,*

1Division of Applied Life Sciences (Brain Korea 21 Program), Gyeongsang National University, Jinju 660-701, Korea, 2Division of Environmental Forest Science, Gyeongsang National University, Jinju 660-701, Korea, 3Institute of Hadong Green Tea, Hadong 667-882, Korea, 4Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea

Correspondence to:*Correspondence: gwcheong@gnu.ac.kr

Received: December 4, 2009; Revised: December 18, 2010; Accepted: December 23, 2009

Abstract

NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to H2O2 or four-electron reduction of oxygen to H2O. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to β-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of β-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce H2O2. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treat-ment with H2O2. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.

Keywords: cell death, dual function, NADH oxidase, protein aggregation, thermophilic archaeon

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

Supplementary File

Share this article on

  • line
  • mail

Molecules and Cells

eISSN 0219-1032
qr-code Download