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Mol. Cells 2010; 29(4): 333-341

Published online March 4, 2010

https://doi.org/10.1007/s10059-010-0029-8

© The Korean Society for Molecular and Cellular Biology

Abelson Virus Transformation Prevents TRAIL Expression by Inhibiting FoxO3a and NF-κB

Mary K. Wilson, Sarah M. McWhirter, Rupesh H. Amin, Dan Huang, and Mark S. Schlissel*

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA

Correspondence to : *Correspondence: mss@berkeley.edu

Received: June 15, 2009; Revised: December 7, 2009; Accepted: December 7, 2009

Abstract

The Abelson Murine Leukemia Virus (A-MuLV) encodes v-Abl, an oncogenic form of the ubiquitous cellular non-receptor tyrosine kinase, c-Abl. A-MuLV specifically transforms murine B cell precursors both in vivo and in vitro. Inhibition of v-Abl by addition of the small molecule inhibitor STI-571 causes these cells to arrest in the G1 phase of the cell cycle prior to undergoing apoptosis. We found that inhibition of v-Abl activity results in upregulation of transcription of the pro-apoptotic TNF-family ligand tumor-necrosis factor-related apoptosis-inducing ligand (TRAIL). Similarly to BCR-Abl-transformed human cells, activation of the transcription factor Foxo3a led to increased TRAIL transcription and induction of a G1 arrest in the absence of v-Abl inhibition, and this effect could be inhibited by the expression of a constitutively active AKT mutant. Multiple pathways act to inhibit FoxO3a activity within Abelson cells. In addition to diminishing transcription factor activity via inhibitory phosphorylation by AKT family members, we found that inhibition of IKK βactivity results in an increase in the total protein level of FoxO3a. Furthermore over-expression of the p65 subunit of NF-κB results in an increase in TRAIL transcription and in apoptosis and deletion of IKK α and β diminishes TRAIL expression and induction. We conclude that in Abelson cells, the inhibition of both NF-κB and FoxO3a activity is required for suppression of TRAIL transcription and maintenance of the transformed state.

Keywords Abelson murine leukemia virus, FoxO3a, NF-κB, pro-B cell leukemia, TRAIL

Article

Research Article

Mol. Cells 2010; 29(4): 333-341

Published online March 4, 2010 https://doi.org/10.1007/s10059-010-0029-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Abelson Virus Transformation Prevents TRAIL Expression by Inhibiting FoxO3a and NF-κB

Mary K. Wilson, Sarah M. McWhirter, Rupesh H. Amin, Dan Huang, and Mark S. Schlissel*

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA

Correspondence to:*Correspondence: mss@berkeley.edu

Received: June 15, 2009; Revised: December 7, 2009; Accepted: December 7, 2009

Abstract

The Abelson Murine Leukemia Virus (A-MuLV) encodes v-Abl, an oncogenic form of the ubiquitous cellular non-receptor tyrosine kinase, c-Abl. A-MuLV specifically transforms murine B cell precursors both in vivo and in vitro. Inhibition of v-Abl by addition of the small molecule inhibitor STI-571 causes these cells to arrest in the G1 phase of the cell cycle prior to undergoing apoptosis. We found that inhibition of v-Abl activity results in upregulation of transcription of the pro-apoptotic TNF-family ligand tumor-necrosis factor-related apoptosis-inducing ligand (TRAIL). Similarly to BCR-Abl-transformed human cells, activation of the transcription factor Foxo3a led to increased TRAIL transcription and induction of a G1 arrest in the absence of v-Abl inhibition, and this effect could be inhibited by the expression of a constitutively active AKT mutant. Multiple pathways act to inhibit FoxO3a activity within Abelson cells. In addition to diminishing transcription factor activity via inhibitory phosphorylation by AKT family members, we found that inhibition of IKK βactivity results in an increase in the total protein level of FoxO3a. Furthermore over-expression of the p65 subunit of NF-κB results in an increase in TRAIL transcription and in apoptosis and deletion of IKK α and β diminishes TRAIL expression and induction. We conclude that in Abelson cells, the inhibition of both NF-κB and FoxO3a activity is required for suppression of TRAIL transcription and maintenance of the transformed state.

Keywords: Abelson murine leukemia virus, FoxO3a, NF-κB, pro-B cell leukemia, TRAIL

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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