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Mol. Cells 2010; 29(3): 239-244

Published online January 21, 2010

https://doi.org/10.1007/s10059-010-0022-2

© The Korean Society for Molecular and Cellular Biology

Proteomic Analysis of Time-Dependant Difference of Protein Expression Profile Changes during Neuronal Differentiation of Mouse Embryonic Stem Cells

Jung Hee Shim1,3, Sun A Cho1,3, Min Ji Seo1, Jung Hee Kim, Na Kyung Ryu1, Kyung Hyun Yoo,Moon Hee Yang1, Seyoon Kim2, Young Yil Bahk2,*, and Jong Hoon Park1,*

1Department of Biological science, Sookmyung Women’s University, Seoul 140-742, Korea, 2Present address: Protein Network Research Center, Yonsei University Seoul 120-749, Korea, 3These authors contributed equally to this work

Correspondence to : *Correspondence: parkjh@sookmyung.ac.kr (JHP); bahk12@empal.com (YYB)

Received: June 22, 2009; Accepted: November 24, 2009

Abstract

The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal develop-ment in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be acti-vated in cultured embryonic stem cells and our two di-mensional electrophoresis data should be useful for investigating some of the mechanism that regulates neu-ronal differentiation.

Keywords 2-DE, differentiation, ES cell, neuronal, proteomic analysis

Article

Research Article

Mol. Cells 2010; 29(3): 239-244

Published online March 31, 2010 https://doi.org/10.1007/s10059-010-0022-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Proteomic Analysis of Time-Dependant Difference of Protein Expression Profile Changes during Neuronal Differentiation of Mouse Embryonic Stem Cells

Jung Hee Shim1,3, Sun A Cho1,3, Min Ji Seo1, Jung Hee Kim, Na Kyung Ryu1, Kyung Hyun Yoo,Moon Hee Yang1, Seyoon Kim2, Young Yil Bahk2,*, and Jong Hoon Park1,*

1Department of Biological science, Sookmyung Women’s University, Seoul 140-742, Korea, 2Present address: Protein Network Research Center, Yonsei University Seoul 120-749, Korea, 3These authors contributed equally to this work

Correspondence to:*Correspondence: parkjh@sookmyung.ac.kr (JHP); bahk12@empal.com (YYB)

Received: June 22, 2009; Accepted: November 24, 2009

Abstract

The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal develop-ment in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be acti-vated in cultured embryonic stem cells and our two di-mensional electrophoresis data should be useful for investigating some of the mechanism that regulates neu-ronal differentiation.

Keywords: 2-DE, differentiation, ES cell, neuronal, proteomic analysis

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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