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Mol. Cells 2010; 29(3): 239-244
Published online January 21, 2010
https://doi.org/10.1007/s10059-010-0022-2
© The Korean Society for Molecular and Cellular Biology
Proteomic Analysis of Time-Dependant Difference of Protein Expression Profile Changes during Neuronal Differentiation of Mouse Embryonic Stem Cells
Correspondence to : *Correspondence: parkjh@sookmyung.ac.kr (JHP); bahk12@empal.com (YYB)
The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal develop-ment in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be acti-vated in cultured embryonic stem cells and our two di-mensional electrophoresis data should be useful for investigating some of the mechanism that regulates neu-ronal differentiation.
Keywords 2-DE, differentiation, ES cell, neuronal, proteomic analysis
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Research Article
Mol. Cells 2010; 29(3): 239-244
Published online March 31, 2010 https://doi.org/10.1007/s10059-010-0022-2
Copyright © The Korean Society for Molecular and Cellular Biology.
Proteomic Analysis of Time-Dependant Difference of Protein Expression Profile Changes during Neuronal Differentiation of Mouse Embryonic Stem Cells
Jung Hee Shim1,3, Sun A Cho1,3, Min Ji Seo1, Jung Hee Kim, Na Kyung Ryu1, Kyung Hyun Yoo,Moon Hee Yang1, Seyoon Kim2, Young Yil Bahk2,*, and Jong Hoon Park1,*
1Department of Biological science, Sookmyung Women’s University, Seoul 140-742, Korea, 2Present address: Protein Network Research Center, Yonsei University Seoul 120-749, Korea, 3These authors contributed equally to this work
Correspondence to:*Correspondence: parkjh@sookmyung.ac.kr (JHP); bahk12@empal.com (YYB)
Abstract
The study of ES cell-mediated neuronal differentiation allows elucidating the mechanism of neuronal develop-ment in spite of the complexity and the difficult accessibility. During the differentiation of embryonic stem cells into neuronal cell, the expression profiles in the level of protein were extensively investigated by proteomic analysis. These cells were analyzed for charges in proteome during the differentiation of ES cells by 2-dimensional electrophoresis (2-DE) and MALDI-TOF MS. Seven unique proteins were identified, some of which were differentially expressed at each stage. A complex system of neuronal differentiation can be acti-vated in cultured embryonic stem cells and our two di-mensional electrophoresis data should be useful for investigating some of the mechanism that regulates neu-ronal differentiation.
Keywords: 2-DE, differentiation, ES cell, neuronal, proteomic analysis
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