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Mol. Cells 2010; 29(1): 77-84

Published online January 31, 2010

https://doi.org/10.1007/s10059-010-0005-3

© The Korean Society for Molecular and Cellular Biology

Identification of an Arabidopsis Nodulin-Related Protein in Heat Stress

Qiantang Fu1,2, Shujia Li1, and Diqiu Yu1,*

1Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China, 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China

Correspondence to : *Correspondence: ydq@xtbg.ac.cn

Received: August 14, 2009; Revised: September 24, 2009; Accepted: September 25, 2009

Abstract

We identified a Nodulin-related protein 1 (NRP1) encoded by At2g03440, which was previously reported to be RPS2 interacting protein in yeast-two-hybrid assay. Northern blotting showed that AtNRP1 expression was suppressed by heat stress (42°C) and induced by low temperature (4°C) treatment. Strong GUS staining was observed in the sites of meristematic tissues of pAtNRP1:: GUS transgenic plants, such as shoot apex and root tips, young leaf veins, stamens and stigmas of flowers, and abscission layers of young siliques. To study AtNRP1 biological functions, we have characterized both loss-of-function T-DNA insertion and transgenic overexpression plants for AtNRP1 in Ara-bidopsis. The T-DNA insertion mutants displayed no obvious difference as compared to wild-type Arabidopsis under heat stress, but the significant enhanced suscepti-bility to heat stress was revealed in two independent AtNRP1-overexpressing transgenic lines. Further study found that the decreased thermtolerance in AtNRP1-overexpressing lines accompanied significantly decreased accumulation of ABA after heat treatment, which was probably due to AtNRP1 playing a role in negative-feedback regulation of the ABA synthesis pathway. These results support the viewpoint that the application of ABA inhibits nodulation and nodulin-related gene expression and threaten adverse ambient temperature can impact the nodulin-related gene expression.

Keywords abscisic acid (ABA), heat stress, nodulin-related protein 1, thermotolerance

Article

Research Article

Mol. Cells 2010; 29(1): 77-84

Published online January 31, 2010 https://doi.org/10.1007/s10059-010-0005-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of an Arabidopsis Nodulin-Related Protein in Heat Stress

Qiantang Fu1,2, Shujia Li1, and Diqiu Yu1,*

1Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China, 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, People’s Republic of China

Correspondence to:*Correspondence: ydq@xtbg.ac.cn

Received: August 14, 2009; Revised: September 24, 2009; Accepted: September 25, 2009

Abstract

We identified a Nodulin-related protein 1 (NRP1) encoded by At2g03440, which was previously reported to be RPS2 interacting protein in yeast-two-hybrid assay. Northern blotting showed that AtNRP1 expression was suppressed by heat stress (42°C) and induced by low temperature (4°C) treatment. Strong GUS staining was observed in the sites of meristematic tissues of pAtNRP1:: GUS transgenic plants, such as shoot apex and root tips, young leaf veins, stamens and stigmas of flowers, and abscission layers of young siliques. To study AtNRP1 biological functions, we have characterized both loss-of-function T-DNA insertion and transgenic overexpression plants for AtNRP1 in Ara-bidopsis. The T-DNA insertion mutants displayed no obvious difference as compared to wild-type Arabidopsis under heat stress, but the significant enhanced suscepti-bility to heat stress was revealed in two independent AtNRP1-overexpressing transgenic lines. Further study found that the decreased thermtolerance in AtNRP1-overexpressing lines accompanied significantly decreased accumulation of ABA after heat treatment, which was probably due to AtNRP1 playing a role in negative-feedback regulation of the ABA synthesis pathway. These results support the viewpoint that the application of ABA inhibits nodulation and nodulin-related gene expression and threaten adverse ambient temperature can impact the nodulin-related gene expression.

Keywords: abscisic acid (ABA), heat stress, nodulin-related protein 1, thermotolerance

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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