Top

Research Article

Split Viewer

Mol. Cells 2008; 25(3): 376-384

Published online May 31, 2008

© The Korean Society for Molecular and Cellular Biology

Glial Fibrillary Acidic Protein Splice Variants in Hepatic Stellate Cells - Expression and Regulation

Michelle Chin Chia Lim, Gunter Maubach and Lang Zhuo

Abstract

The glial fibrillary acidic protein (GFAP) is traditionally used as a marker for astrocytes of the brain, and more recently for the hepatic stellate cells (HSCs) of the liver. Several GFAP splice variants have been previously reported in the astrocytes of the CNS and in the non-myelinating Schwann cells of the PNS. In this study, we investigate whether GFAP splice variants are present in the HSCs and their expression as a function of HSCs activation. Furthermore, the regulation of these transcripts upon treatment with interferon gamma (IFN-γ) will be explored. Using semi-quan-titative RT-PCR and real-time PCR, we examine the expression and regulation of GFAP splice variants in HSCs as well as their respective half-life. We discover that most of the GFAP splice variants (GFAPα, β, δ, ε and k) found in the neural system are also expressed in quiescent and culture-activated primary HSCs. Interestingly, GFAPα is the predominant form in quiescent and culture-activated primary HSCs, while GFAP? predominates in the SV40-immortalized activated HSC-T6. GFAPδ, ε and ? have similar half-lives of 10 hours, while GFAPβ has a half-life of 17 hours. Treatment of HSC-T6 with IFN-γ results in a significant 1.29-fold up-regulation of GFAPα whereas the level of the other transcripts remains unchanged. In summary, GFAPα, β, δ, ε and k are present in HSCs. They are differentially regulated on the transcription level, implying a role of the 5’ and 3’ untranslated regions.

Keywords Expression, GFAP Splice Variants, Hepatic Stellate Cells, IFN-gamma

Article

Research Article

Mol. Cells 2008; 25(3): 376-384

Published online May 31, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Glial Fibrillary Acidic Protein Splice Variants in Hepatic Stellate Cells - Expression and Regulation

Michelle Chin Chia Lim, Gunter Maubach and Lang Zhuo

Abstract

The glial fibrillary acidic protein (GFAP) is traditionally used as a marker for astrocytes of the brain, and more recently for the hepatic stellate cells (HSCs) of the liver. Several GFAP splice variants have been previously reported in the astrocytes of the CNS and in the non-myelinating Schwann cells of the PNS. In this study, we investigate whether GFAP splice variants are present in the HSCs and their expression as a function of HSCs activation. Furthermore, the regulation of these transcripts upon treatment with interferon gamma (IFN-γ) will be explored. Using semi-quan-titative RT-PCR and real-time PCR, we examine the expression and regulation of GFAP splice variants in HSCs as well as their respective half-life. We discover that most of the GFAP splice variants (GFAPα, β, δ, ε and k) found in the neural system are also expressed in quiescent and culture-activated primary HSCs. Interestingly, GFAPα is the predominant form in quiescent and culture-activated primary HSCs, while GFAP? predominates in the SV40-immortalized activated HSC-T6. GFAPδ, ε and ? have similar half-lives of 10 hours, while GFAPβ has a half-life of 17 hours. Treatment of HSC-T6 with IFN-γ results in a significant 1.29-fold up-regulation of GFAPα whereas the level of the other transcripts remains unchanged. In summary, GFAPα, β, δ, ε and k are present in HSCs. They are differentially regulated on the transcription level, implying a role of the 5’ and 3’ untranslated regions.

Keywords: Expression, GFAP Splice Variants, Hepatic Stellate Cells, IFN-gamma

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download