Top

Research Article

Split Viewer

Mol. Cells 2009; 28(6): 521-527

Published online November 19, 2009

https://doi.org/10.1007/s10059-009-0158-0

© The Korean Society for Molecular and Cellular Biology

miR-372 Regulates Cell Cycle and Apoptosis of AGS Human Gastric Cancer Cell Line through Direct Regulation of LATS2

Wha Ja Cho, Jeong Min Shin, Jong Soo Kim, Man Ryul Lee, Ki Sung Hong, Jun-Ho Lee,
Kyoung Hwa Koo, Jeong Woo Park, and Kye-Seong Kim

Received: May 21, 2009; Revised: September 22, 2009; Accepted: September 22, 2009

Abstract

Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.

Keywords AGS gastric cancer cell line, apoptosis, cell cycle, G2-M transition, LATS2, miR-372

Article

Research Article

Mol. Cells 2009; 28(6): 521-527

Published online December 31, 2009 https://doi.org/10.1007/s10059-009-0158-0

Copyright © The Korean Society for Molecular and Cellular Biology.

miR-372 Regulates Cell Cycle and Apoptosis of AGS Human Gastric Cancer Cell Line through Direct Regulation of LATS2

Wha Ja Cho, Jeong Min Shin, Jong Soo Kim, Man Ryul Lee, Ki Sung Hong, Jun-Ho Lee,
Kyoung Hwa Koo, Jeong Woo Park, and Kye-Seong Kim

Received: May 21, 2009; Revised: September 22, 2009; Accepted: September 22, 2009

Abstract

Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.

Keywords: AGS gastric cancer cell line, apoptosis, cell cycle, G2-M transition, LATS2, miR-372

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

Supplementary File

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download