Mol. Cells 2009; 28(6): 509-513
Published online December 31, 2009
https://doi.org/10.1007/s10059-009-0160-6
© The Korean Society for Molecular and Cellular Biology
Here, we show that JNK1 and JNK3 have different roles in TNF-α- or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited TNF-α- or etoposide-induced apoptosis, while dominant negative JNK3 promoted TNF-α- or etoposide-induced apoptosis. During TNF-α-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in TNF-α-induced apoptosis originates before the apoptotic machinery is triggered.
Keywords : anti-apoptotic, apoptosis, etoposide, JNK1, JNK3, survival, TNF-α
Mol. Cells 2009; 28(6): 509-513
Published online December 31, 2009 https://doi.org/10.1007/s10059-009-0160-6
Copyright © The Korean Society for Molecular and Cellular Biology.
Young-Mi Ham, Jin-Hee Lim, and Seung-Ki Lee
Here, we show that JNK1 and JNK3 have different roles in TNF-α- or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited TNF-α- or etoposide-induced apoptosis, while dominant negative JNK3 promoted TNF-α- or etoposide-induced apoptosis. During TNF-α-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in TNF-α-induced apoptosis originates before the apoptotic machinery is triggered.
Keywords: : anti-apoptotic, apoptosis, etoposide, JNK1, JNK3, survival, TNF-&alpha,
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