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Mol. Cells 2009; 28(6): 509-513

Published online December 31, 2009

https://doi.org/10.1007/s10059-009-0160-6

© The Korean Society for Molecular and Cellular Biology

Distinct Roles for JNK1 and JNK3 During TNF-α- or
Etoposide-Induced Apoptosis in HeLa Cells

Young-Mi Ham, Jin-Hee Lim, and Seung-Ki Lee

Received: December 17, 2009; Revised: October 11, 2009; Accepted: October 12, 2009

Abstract

Here, we show that JNK1 and JNK3 have different roles in TNF-α- or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited TNF-α- or etoposide-induced apoptosis, while dominant negative JNK3 promoted TNF-α- or etoposide-induced apoptosis. During TNF-α-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in TNF-α-induced apoptosis originates before the apoptotic machinery is triggered.

Keywords : anti-apoptotic, apoptosis, etoposide, JNK1, JNK3, survival, TNF-α

Article

Research Article

Mol. Cells 2009; 28(6): 509-513

Published online December 31, 2009 https://doi.org/10.1007/s10059-009-0160-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Distinct Roles for JNK1 and JNK3 During TNF-α- or
Etoposide-Induced Apoptosis in HeLa Cells

Young-Mi Ham, Jin-Hee Lim, and Seung-Ki Lee

Received: December 17, 2009; Revised: October 11, 2009; Accepted: October 12, 2009

Abstract

Here, we show that JNK1 and JNK3 have different roles in TNF-α- or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited TNF-α- or etoposide-induced apoptosis, while dominant negative JNK3 promoted TNF-α- or etoposide-induced apoptosis. During TNF-α-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in TNF-α-induced apoptosis originates before the apoptotic machinery is triggered.

Keywords: : anti-apoptotic, apoptosis, etoposide, JNK1, JNK3, survival, TNF-&alpha,

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

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