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Mol. Cells 2009; 28(5): 489-494

Published online October 21, 2009

https://doi.org/10.1007/s10059-009-0141-9

© The Korean Society for Molecular and Cellular Biology

The p53-p21Cip1/WAF1 Pathway Is Necessary forCellular Senescence Induced by the Inhibition of Protein Kinase CKII in Human Colon Cancer Cells

Ji-Young Kang, Jin Joo Kim, Seok Young Jang, and Young-Seuk Bae

Received: May 6, 2009; Revised: August 20, 2009; Accepted: September 7, 2009

Abstract

We have previously shown that the down-regulation of protein kinase CKII activity is tightly associated with cellular senescence of human fibroblast IMR-90 cells. Here, we examined the roles of p53 and p21Cip1/WAF1 in senescence development induced by CKII inhibition using wild-type, isogenic p53-/- and isogenic p21-/- HCT116 human colon cancer cell lines. A senescent marker appeared after staining for senescence-associated β-galactosidase activity in wild-type HCT116 cells treated with CKII inhibitor or CKIIα siRNA, but this response was almost abolished in p53- or p21Cip1/WAF1-null cells. Increased cellular levels of p53 and p21Cip1/WAF1 protein occurred with the inhibition of CKII. CKII inhibition upregulated p53 and p21Cip1/WAF1 expression at post-transcriptional level and transcription level, respec-tively. RB phosphorylation significantly decreased in cells treated with CKII inhibitor. Taken together, this study shows that the activation of the p53-p21Cip1/WAF1 pathway acts as a major mediator of cellular senescence induced by CKII inhibition.

Keywords human colon cancer cell, p21Cip1/WAF1, p53, protein kinase CKII, senescence

Article

Communication

Mol. Cells 2009; 28(5): 489-494

Published online November 30, 2009 https://doi.org/10.1007/s10059-009-0141-9

Copyright © The Korean Society for Molecular and Cellular Biology.

The p53-p21Cip1/WAF1 Pathway Is Necessary forCellular Senescence Induced by the Inhibition of Protein Kinase CKII in Human Colon Cancer Cells

Ji-Young Kang, Jin Joo Kim, Seok Young Jang, and Young-Seuk Bae

Received: May 6, 2009; Revised: August 20, 2009; Accepted: September 7, 2009

Abstract

We have previously shown that the down-regulation of protein kinase CKII activity is tightly associated with cellular senescence of human fibroblast IMR-90 cells. Here, we examined the roles of p53 and p21Cip1/WAF1 in senescence development induced by CKII inhibition using wild-type, isogenic p53-/- and isogenic p21-/- HCT116 human colon cancer cell lines. A senescent marker appeared after staining for senescence-associated β-galactosidase activity in wild-type HCT116 cells treated with CKII inhibitor or CKIIα siRNA, but this response was almost abolished in p53- or p21Cip1/WAF1-null cells. Increased cellular levels of p53 and p21Cip1/WAF1 protein occurred with the inhibition of CKII. CKII inhibition upregulated p53 and p21Cip1/WAF1 expression at post-transcriptional level and transcription level, respec-tively. RB phosphorylation significantly decreased in cells treated with CKII inhibitor. Taken together, this study shows that the activation of the p53-p21Cip1/WAF1 pathway acts as a major mediator of cellular senescence induced by CKII inhibition.

Keywords: human colon cancer cell, p21Cip1/WAF1, p53, protein kinase CKII, senescence

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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