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Mol. Cells 2009; 28(5): 463-472

Published online October 21, 2009

https://doi.org/10.1007/s10059-009-0138-4

© The Korean Society for Molecular and Cellular Biology

Structure and Expression of OsUBP6, an
Ubiquitin-Specific Protease 6 Homolog in Rice
(Oryza sativa L.)

Yea Kyung Moon, Jong-Pil Hong, Young-Chan Cho, Sae-Jun Yang, Gynheung An, and
Woo Taek Kim

Received: July 30, 2009; Revised: August 27, 2009; Accepted: September 2, 2009

Abstract

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of devel-opment in rice plants.

Keywords gene expression, rice (Oryza sativa L.), T-DNA knock-out mutant, ubiquitin, ubiquitin-specific proteases

Article

Research Article

Mol. Cells 2009; 28(5): 463-472

Published online November 30, 2009 https://doi.org/10.1007/s10059-009-0138-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Structure and Expression of OsUBP6, an
Ubiquitin-Specific Protease 6 Homolog in Rice
(Oryza sativa L.)

Yea Kyung Moon, Jong-Pil Hong, Young-Chan Cho, Sae-Jun Yang, Gynheung An, and
Woo Taek Kim

Received: July 30, 2009; Revised: August 27, 2009; Accepted: September 2, 2009

Abstract

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of devel-opment in rice plants.

Keywords: gene expression, rice (Oryza sativa L.), T-DNA knock-out mutant, ubiquitin, ubiquitin-specific proteases

Mol. Cells
Jun 30, 2023 Vol.46 No.6, pp. 329~398
COVER PICTURE
The cellular proteostasis network is adaptively modulated upon cellular stress, thereby protecting cells from proteostasis collapse. Heat shock induces the translocation of misfolded proteins and the chaperone protein HSP70 into nucleolus, where nuclear protein quality control primarily occurs. Nuclear RNA export factor 1 (green), nucleolar protein fibrillarin (red), and nuclei (blue) were visualized in NIH3T3 cells under basal (left) and heat shock (right) conditions (Park et al., pp. 374-386).

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