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Mol. Cells 2009; 28(5): 431-439

Published online October 29, 2009

https://doi.org/10.1007/s10059-009-0161-5

© The Korean Society for Molecular and Cellular Biology

Rice OsACDR1 (Oryza sativa Accelerated Cell
Death and Resistance 1) Is a Potential Positive
Regulator of Fungal Disease Resistance

Jung-A Kim, Kyoungwon Cho, Raksha Singh, Young-Ho Jung, Seung-Hee Jeong, So-Hee Kim,
Jae-eun Lee, Yoon-Seong Cho, Ganesh K. Agrawal, Randeep Rakwal, Shigeru Tamogami,
Birgit Kersten, Jong-Seong Jeon, Gynheung An, and Nam-Soo Jwa

Received: July 3, 2009; Revised: October 2, 2009; Accepted: October 8, 2009

Abstract

Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.

Keywords disease resistance, lesion, MAPKKK, OsACDR1, pathogen, rice

Article

Research Article

Mol. Cells 2009; 28(5): 431-439

Published online November 30, 2009 https://doi.org/10.1007/s10059-009-0161-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Rice OsACDR1 (Oryza sativa Accelerated Cell
Death and Resistance 1) Is a Potential Positive
Regulator of Fungal Disease Resistance

Jung-A Kim, Kyoungwon Cho, Raksha Singh, Young-Ho Jung, Seung-Hee Jeong, So-Hee Kim,
Jae-eun Lee, Yoon-Seong Cho, Ganesh K. Agrawal, Randeep Rakwal, Shigeru Tamogami,
Birgit Kersten, Jong-Seong Jeon, Gynheung An, and Nam-Soo Jwa

Received: July 3, 2009; Revised: October 2, 2009; Accepted: October 8, 2009

Abstract

Rice Oryza sativa accelerated cell death and resistance 1 (OsACDR1) encodes a putative Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of-function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of-function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1:GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDR1 plays an important role in the positive regulation of disease resistance in rice.

Keywords: disease resistance, lesion, MAPKKK, OsACDR1, pathogen, rice

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

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