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Mol. Cells 2009; 28(5): 417-422

Published online November 30, 2009

https://doi.org/10.1007/s10059-009-0137-5

© The Korean Society for Molecular and Cellular Biology

Rhythmic Expression of Mitogen Activated Protein
Kinase Activity in Rice

Kudupudi Prabhakara Rao, Gubbala Vani, Kundan Kumar, and Alok Krishna Sinha

Received: June 1, 2009; Revised: August 12, 2009; Accepted: August 26, 2009

Abstract

Mitogen activated protein kinase (MAPK) are known to get activated during various stress signals and transduce the message from the cell membrane to the nucleus for appropriate cellular reorganization. Though, a certain basal activity of MAPK is often observed in the control plants. Prolonged exposure of rice plants to lowered or elevated temperature exhibited a rhythm in the activation of MAPKs. We analyzed existence of a possible endogenous rhythm in the activity of MAPKs in rice plants. The plants growing at constant temperature entrained in 16/8 h day-night cycle showed diurnal rhythm in activity. When the activation of MAPK was tested under continuous conditions by shifting plants to continuous darkness for a period of 72 h, the periodic rhythm persisted and followed a circadian pattern. Analysis of the transcripts of group A, B and C members of MAPKs under above conditions by quantitative real time PCR revealed that the members of group C exhibit periodic rhythm. Our data indicates that the MAP kinase activity in rice follows rhythmic expression in a circadian manner.

Keywords circadian clock, diurnal rhythm, mitogen activated protein kinase (MAPK), rice, signal transduction

Article

Research Article

Mol. Cells 2009; 28(5): 417-422

Published online November 30, 2009 https://doi.org/10.1007/s10059-009-0137-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Rhythmic Expression of Mitogen Activated Protein
Kinase Activity in Rice

Kudupudi Prabhakara Rao, Gubbala Vani, Kundan Kumar, and Alok Krishna Sinha

Received: June 1, 2009; Revised: August 12, 2009; Accepted: August 26, 2009

Abstract

Mitogen activated protein kinase (MAPK) are known to get activated during various stress signals and transduce the message from the cell membrane to the nucleus for appropriate cellular reorganization. Though, a certain basal activity of MAPK is often observed in the control plants. Prolonged exposure of rice plants to lowered or elevated temperature exhibited a rhythm in the activation of MAPKs. We analyzed existence of a possible endogenous rhythm in the activity of MAPKs in rice plants. The plants growing at constant temperature entrained in 16/8 h day-night cycle showed diurnal rhythm in activity. When the activation of MAPK was tested under continuous conditions by shifting plants to continuous darkness for a period of 72 h, the periodic rhythm persisted and followed a circadian pattern. Analysis of the transcripts of group A, B and C members of MAPKs under above conditions by quantitative real time PCR revealed that the members of group C exhibit periodic rhythm. Our data indicates that the MAP kinase activity in rice follows rhythmic expression in a circadian manner.

Keywords: circadian clock, diurnal rhythm, mitogen activated protein kinase (MAPK), rice, signal transduction

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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