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Mol. Cells 2009; 28(4): 389-395

Published online October 31, 2009

https://doi.org/10.1007/s10059-009-0133-9

© The Korean Society for Molecular and Cellular Biology

A tdcA Mutation Reduces the Invasive Ability of Salmonella enterica Serovar Typhimurium

Minjeong Kim, Sangyong Lim, Dongho Kim, Hyon E. Choy, and Sangryeol Ryu

Received: July 13, 2009; Revised: August 25, 2009; Accepted: August 26, 2009

Abstract

We previously observed that the transcription of some flagellar genes decreased in Salmonella Typhimurium tdcA mutant, which is a gene encoding the transcriptional activator of the tdc operon. Since flagella-mediated bacterial motility accelerates the invasion of Salmonella, we have examined the effect of tdcA mutation on the invasive ability as well as the flagellar biosynthesis in S. Typhimurium. A tdcA mutation caused defects in motility and formation of flagellin protein, FliC in S. Typhimurium. Invasion assays in the presence of a centrifugal force confirmed that the defect of flagellum synthesis decreases the ability of Salmonella to invade into cultured epithelial cells. In addition, we also found that the expression of Salmonella pathogenicity island 1 (SPI1) genes required for Salmonella invasion was down-regulated in the tdcA mutant because of the decreased expression of fliZ, a positive regulator of SPI1 transcriptional activator, hilA. Finally, the virulence of a S. Typhimurium tdcA mutant was attenuated compared to a wild type when administered orally. This study implies the role of tdcA in the invasion process of S. Typhimurium.

Keywords flagella, invasion, Salmonella pathogenicity island, tdcA

Article

Research Article

Mol. Cells 2009; 28(4): 389-395

Published online October 31, 2009 https://doi.org/10.1007/s10059-009-0133-9

Copyright © The Korean Society for Molecular and Cellular Biology.

A tdcA Mutation Reduces the Invasive Ability of Salmonella enterica Serovar Typhimurium

Minjeong Kim, Sangyong Lim, Dongho Kim, Hyon E. Choy, and Sangryeol Ryu

Received: July 13, 2009; Revised: August 25, 2009; Accepted: August 26, 2009

Abstract

We previously observed that the transcription of some flagellar genes decreased in Salmonella Typhimurium tdcA mutant, which is a gene encoding the transcriptional activator of the tdc operon. Since flagella-mediated bacterial motility accelerates the invasion of Salmonella, we have examined the effect of tdcA mutation on the invasive ability as well as the flagellar biosynthesis in S. Typhimurium. A tdcA mutation caused defects in motility and formation of flagellin protein, FliC in S. Typhimurium. Invasion assays in the presence of a centrifugal force confirmed that the defect of flagellum synthesis decreases the ability of Salmonella to invade into cultured epithelial cells. In addition, we also found that the expression of Salmonella pathogenicity island 1 (SPI1) genes required for Salmonella invasion was down-regulated in the tdcA mutant because of the decreased expression of fliZ, a positive regulator of SPI1 transcriptional activator, hilA. Finally, the virulence of a S. Typhimurium tdcA mutant was attenuated compared to a wild type when administered orally. This study implies the role of tdcA in the invasion process of S. Typhimurium.

Keywords: flagella, invasion, Salmonella pathogenicity island, tdcA

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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