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Mol. Cells 2009; 28(4): 369-373

Published online September 30, 2009

https://doi.org/10.1007/s10059-009-0131-y

© The Korean Society for Molecular and Cellular Biology

Production of Cellulosic Ethanol inSaccharomyces cerevisiae Heterologous Express-ing Clostridium thermocellum Endoglucanase andSaccharomycopsis fibuligera ß-glucosidase Genes

Eugene Jeon, Jeong-eun Hyeon, Dong Jin Suh, Young-Woong Suh, Seoung Wook Kim,
Kwang Ho Song, and Sung Ok Han

Received: June 15, 2009; Revised: July 23, 2009; Accepted: August 17, 2009

Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an
β-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Keywords beta-glucosidase, cellulose degradation, Clostridium thermocellum, endoglucanase, ethanol production, extracellular expression, Saccha-romyces cerevisiae

Article

Research Article

Mol. Cells 2009; 28(4): 369-373

Published online October 31, 2009 https://doi.org/10.1007/s10059-009-0131-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Production of Cellulosic Ethanol inSaccharomyces cerevisiae Heterologous Express-ing Clostridium thermocellum Endoglucanase andSaccharomycopsis fibuligera ß-glucosidase Genes

Eugene Jeon, Jeong-eun Hyeon, Dong Jin Suh, Young-Woong Suh, Seoung Wook Kim,
Kwang Ho Song, and Sung Ok Han

Received: June 15, 2009; Revised: July 23, 2009; Accepted: August 17, 2009

Abstract

Heterologous secretory expression of endoglucanase E (Clostridium thermocellum) and β-glucosidase 1 (Saccharomycopsis fibuligera) was achieved in Saccharomyces cerevisiae fermentation cultures as an
β-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.

Keywords: beta-glucosidase, cellulose degradation, Clostridium thermocellum, endoglucanase, ethanol production, extracellular expression, Saccha-romyces cerevisiae

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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