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Mol. Cells 2009; 28(3): 183-188

Published online September 30, 2009

https://doi.org/10.1007/s10059-009-0121-0

© The Korean Society for Molecular and Cellular Biology

The Effector Functions of Mature T Lympho-cytes
Are Impaired in Transgenic Mice Expressing the
SH2 Domain of TSAd/Lad

Youngbong Choi, Eunkyung Park, Eunseon Ahn, Inyoung Park, and Yungdae Yun

Received: April 21, 2009; Revised: July 20, 2009; Accepted: July 27, 2009

Abstract

TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.

Keywords adhesion, Lad, migration, SH2 domain, TSAd

Article

Research Article

Mol. Cells 2009; 28(3): 183-188

Published online September 30, 2009 https://doi.org/10.1007/s10059-009-0121-0

Copyright © The Korean Society for Molecular and Cellular Biology.

The Effector Functions of Mature T Lympho-cytes
Are Impaired in Transgenic Mice Expressing the
SH2 Domain of TSAd/Lad

Youngbong Choi, Eunkyung Park, Eunseon Ahn, Inyoung Park, and Yungdae Yun

Received: April 21, 2009; Revised: July 20, 2009; Accepted: July 27, 2009

Abstract

TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.

Keywords: adhesion, Lad, migration, SH2 domain, TSAd

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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