Mol. Cells 2009; 28(3): 183-188
Published online September 4, 2009
https://doi.org/10.1007/s10059-009-0121-0
© The Korean Society for Molecular and Cellular Biology
TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.
Keywords adhesion, Lad, migration, SH2 domain, TSAd
Mol. Cells 2009; 28(3): 183-188
Published online September 30, 2009 https://doi.org/10.1007/s10059-009-0121-0
Copyright © The Korean Society for Molecular and Cellular Biology.
Youngbong Choi, Eunkyung Park, Eunseon Ahn, Inyoung Park, and Yungdae Yun
TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.
Keywords: adhesion, Lad, migration, SH2 domain, TSAd
Se-Jeong Park, Ho-Yeon Song, and Hyung-Sun Youn
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