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Mol. Cells 2009; 28(3): 183-188

Published online September 4, 2009

https://doi.org/10.1007/s10059-009-0121-0

© The Korean Society for Molecular and Cellular Biology

The Effector Functions of Mature T Lympho-cytes
Are Impaired in Transgenic Mice Expressing the
SH2 Domain of TSAd/Lad

Youngbong Choi, Eunkyung Park, Eunseon Ahn, Inyoung Park, and Yungdae Yun

Received: April 21, 2009; Revised: July 20, 2009; Accepted: July 27, 2009

Abstract

TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.

Keywords adhesion, Lad, migration, SH2 domain, TSAd

Article

Research Article

Mol. Cells 2009; 28(3): 183-188

Published online September 30, 2009 https://doi.org/10.1007/s10059-009-0121-0

Copyright © The Korean Society for Molecular and Cellular Biology.

The Effector Functions of Mature T Lympho-cytes
Are Impaired in Transgenic Mice Expressing the
SH2 Domain of TSAd/Lad

Youngbong Choi, Eunkyung Park, Eunseon Ahn, Inyoung Park, and Yungdae Yun

Received: April 21, 2009; Revised: July 20, 2009; Accepted: July 27, 2009

Abstract

TSAd/Lad is a T cell adaptor molecule involved in p56lck-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice ex-pressing the SH2 domain of TSAd (TSAd-SH2) under the control of the p56lck proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as Ca2+ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.

Keywords: adhesion, Lad, migration, SH2 domain, TSAd

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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