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Mol. Cells 2009; 28(2): 99-103

Published online August 31, 2009

https://doi.org/10.1007/s10059-009-0107-y

© The Korean Society for Molecular and Cellular Biology

Structural and Quantitative Expression Analyses of
HERV Gene Family in Human Tissues

Kung Ahn, and Heui-Soo Kim

Received: April 30, 2009; Revised: June 11, 2009; Accepted: June 24, 2009

Abstract

Human endogenous retroviruses (HERVs) have been implicated in the pathogenesis of several human dis-eases as multi-copy members in the human genome. Their gene expression profiling could provide us with important insights into the pathogenic relationship between HERVs and cancer. In this study, we have evalu-ated the genomic structure and quantitatively determined the expression patterns in the env gene of a variety of HERV family members located on six specific loci by the RetroTector 10 program, as well as real-time RT-PCR amplification. The env gene transcripts evidenced significant differences in the human tumor/normal adjacent tissues (colon, liver, uterus, lung and testis). As compared to the adjacent normal tissues, high levels of expression were noted in testis tumor tissues for HERV-K, in liver and lung tumor tissues for HERV-R, in liver, lung, and testis tumor tissues for HERV-H, and in colon and liver tumor tissues for HERV-P. These data warrant further studies with larger groups of patients to develop biomarkers for specific human cancers.

Keywords biomarker, HERV env gene, real-time RT-PCR, tumor tissues

Article

Research Article

Mol. Cells 2009; 28(2): 99-103

Published online August 31, 2009 https://doi.org/10.1007/s10059-009-0107-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Structural and Quantitative Expression Analyses of
HERV Gene Family in Human Tissues

Kung Ahn, and Heui-Soo Kim

Received: April 30, 2009; Revised: June 11, 2009; Accepted: June 24, 2009

Abstract

Human endogenous retroviruses (HERVs) have been implicated in the pathogenesis of several human dis-eases as multi-copy members in the human genome. Their gene expression profiling could provide us with important insights into the pathogenic relationship between HERVs and cancer. In this study, we have evalu-ated the genomic structure and quantitatively determined the expression patterns in the env gene of a variety of HERV family members located on six specific loci by the RetroTector 10 program, as well as real-time RT-PCR amplification. The env gene transcripts evidenced significant differences in the human tumor/normal adjacent tissues (colon, liver, uterus, lung and testis). As compared to the adjacent normal tissues, high levels of expression were noted in testis tumor tissues for HERV-K, in liver and lung tumor tissues for HERV-R, in liver, lung, and testis tumor tissues for HERV-H, and in colon and liver tumor tissues for HERV-P. These data warrant further studies with larger groups of patients to develop biomarkers for specific human cancers.

Keywords: biomarker, HERV env gene, real-time RT-PCR, tumor tissues

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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