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Mol. Cells 2009; 28(2): 93-98

Published online July 30, 2009

https://doi.org/10.1007/s10059-009-0105-0

© The Korean Society for Molecular and Cellular Biology

Overexpression of the Downward Leaf Curling
(DLC) Gene from Melon Changes Leaf Morphol-ogy
by Controlling Cell Size and Shape in Arabidopsis
Leaves

Jae-Jun Kee, Sang Eun Jun, Seung-A Baek, Tae-Soo Lee, Myung Rae Cho, Hyun-Sik Hwang,
Suk-Chan Lee, Jongkee Kim , Gyung-Tae Kim, and Kyung-Hoan Im

Received: April 30, 2009; Revised: June 10, 2009; Accepted: June 15, 2009

Abstract

A plant-specific gene was cloned from melon fruit. This gene was named downward leaf curling (CmDLC) based on the phenotype of transgenic Arabidopsis plants overexpressing the gene. This expression level of this gene was especially upregulated during melon fruit enlargement. Overexpression of CmDLC in Arabidopsis resulted in dwarfism and narrow, epinastically curled leaves. These phenotypes were found to be caused by a reduction in cell number and cell size on the adaxial and abaxial sides of the epidermis, with a greater reduction on the abaxial side of the leaves. These phenotypic characteristics, combined with the more wavy morphology of epidermal cells in overexpression lines, indicate that CmDLC overexpression affects cell elongation and cell morphology. To investigate intracellular protein localization, a CmDLC-GFP fusion protein was made and expressed in onion epidermal cells. This protein was observed to be preferentially localized close to the cell membrane. Thus, we report here a new plant-specific gene that is localized to the cell membrane and that controls leaf cell number, size and morphology.

Keywords Arabidopsis, cell size, CmDLC, epinasty, leaf morphology, melon

Article

Research Article

Mol. Cells 2009; 28(2): 93-98

Published online August 31, 2009 https://doi.org/10.1007/s10059-009-0105-0

Copyright © The Korean Society for Molecular and Cellular Biology.

Overexpression of the Downward Leaf Curling
(DLC) Gene from Melon Changes Leaf Morphol-ogy
by Controlling Cell Size and Shape in Arabidopsis
Leaves

Jae-Jun Kee, Sang Eun Jun, Seung-A Baek, Tae-Soo Lee, Myung Rae Cho, Hyun-Sik Hwang,
Suk-Chan Lee, Jongkee Kim , Gyung-Tae Kim, and Kyung-Hoan Im

Received: April 30, 2009; Revised: June 10, 2009; Accepted: June 15, 2009

Abstract

A plant-specific gene was cloned from melon fruit. This gene was named downward leaf curling (CmDLC) based on the phenotype of transgenic Arabidopsis plants overexpressing the gene. This expression level of this gene was especially upregulated during melon fruit enlargement. Overexpression of CmDLC in Arabidopsis resulted in dwarfism and narrow, epinastically curled leaves. These phenotypes were found to be caused by a reduction in cell number and cell size on the adaxial and abaxial sides of the epidermis, with a greater reduction on the abaxial side of the leaves. These phenotypic characteristics, combined with the more wavy morphology of epidermal cells in overexpression lines, indicate that CmDLC overexpression affects cell elongation and cell morphology. To investigate intracellular protein localization, a CmDLC-GFP fusion protein was made and expressed in onion epidermal cells. This protein was observed to be preferentially localized close to the cell membrane. Thus, we report here a new plant-specific gene that is localized to the cell membrane and that controls leaf cell number, size and morphology.

Keywords: Arabidopsis, cell size, CmDLC, epinasty, leaf morphology, melon

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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