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Mol. Cells 2009; 28(2): 87-92

Published online August 20, 2009

https://doi.org/10.1007/s10059-009-0110-3

© The Korean Society for Molecular and Cellular Biology

Identification of GATA2 and AP-1 Activator
Elements within the Enhancer VNTR Occurring in
Intron 5 of the Human SIRT3 Gene

Dina Bellizzi, Giuseppina Covello, Fausta Di Cianni, Qiang Tong, and Giovanna De Benedictis

Received: April 1, 2009; Revised: June 12, 2009; Accepted: June 30, 2009

Abstract

Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T > C transition of the second VNTR repeat affects their activity.

Keywords AP-1 site, enhancer VNTR, GATA2 site, SIRT3 gene

Article

Research Article

Mol. Cells 2009; 28(2): 87-92

Published online August 31, 2009 https://doi.org/10.1007/s10059-009-0110-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of GATA2 and AP-1 Activator
Elements within the Enhancer VNTR Occurring in
Intron 5 of the Human SIRT3 Gene

Dina Bellizzi, Giuseppina Covello, Fausta Di Cianni, Qiang Tong, and Giovanna De Benedictis

Received: April 1, 2009; Revised: June 12, 2009; Accepted: June 30, 2009

Abstract

Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T > C transition of the second VNTR repeat affects their activity.

Keywords: AP-1 site, enhancer VNTR, GATA2 site, SIRT3 gene

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
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Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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