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Mol. Cells 2009; 28(2): 87-92

Published online August 20, 2009

https://doi.org/10.1007/s10059-009-0110-3

© The Korean Society for Molecular and Cellular Biology

Identification of GATA2 and AP-1 Activator
Elements within the Enhancer VNTR Occurring in
Intron 5 of the Human SIRT3 Gene

Dina Bellizzi, Giuseppina Covello, Fausta Di Cianni, Qiang Tong, and Giovanna De Benedictis

Received: April 1, 2009; Revised: June 12, 2009; Accepted: June 30, 2009

Abstract

Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T > C transition of the second VNTR repeat affects their activity.

Keywords AP-1 site, enhancer VNTR, GATA2 site, SIRT3 gene

Article

Research Article

Mol. Cells 2009; 28(2): 87-92

Published online August 31, 2009 https://doi.org/10.1007/s10059-009-0110-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of GATA2 and AP-1 Activator
Elements within the Enhancer VNTR Occurring in
Intron 5 of the Human SIRT3 Gene

Dina Bellizzi, Giuseppina Covello, Fausta Di Cianni, Qiang Tong, and Giovanna De Benedictis

Received: April 1, 2009; Revised: June 12, 2009; Accepted: June 30, 2009

Abstract

Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T > C transition of the second VNTR repeat affects their activity.

Keywords: AP-1 site, enhancer VNTR, GATA2 site, SIRT3 gene

Mol. Cells
Mar 31, 2023 Vol.46 No.3, pp. 131~189
COVER PICTURE
The physiologically important cytoprotective signaling in normal cells (background area in turquoise) mediated by NRF2 (blue chain) is often hijacked by cancer cells (red ball) in the tumor microenvironment (yellow area). However, the differential roles of NRF2 throughout the multistage carcinogenesis remains largely unresolved (white-colored overlapping misty areas).

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