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Mol. Cells 2009; 27(5): 609-613

Published online May 15, 2009

https://doi.org/10.1007/s10059-009-0074-3

© The Korean Society for Molecular and Cellular Biology

Antioxidative Role of Selenoprotein W in Oxi-dant-Induced Mouse Embryonic Neuronal Cell Death

Youn Wook Chung, Daewon Jeong, Ok Jeong Noh, Yong Hwan Park, Soo Im Kang, Min Goo Lee, Tae-Hoon Lee, Moon Bin Yim, and Ick Young Kim

Received: February 23, 2009; Accepted: March 6, 2009

Abstract

It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

Keywords antioxidant, neuronal cells, oxidative stress, selenium, selenoprotein W

Article

Research Article

Mol. Cells 2009; 27(5): 609-613

Published online May 31, 2009 https://doi.org/10.1007/s10059-009-0074-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Antioxidative Role of Selenoprotein W in Oxi-dant-Induced Mouse Embryonic Neuronal Cell Death

Youn Wook Chung, Daewon Jeong, Ok Jeong Noh, Yong Hwan Park, Soo Im Kang, Min Goo Lee, Tae-Hoon Lee, Moon Bin Yim, and Ick Young Kim

Received: February 23, 2009; Accepted: March 6, 2009

Abstract

It has been reported that selenoprotein W (SelW) mRNA is highly expressed in the developing central nerve system of rats, and its expression is maintained until the early postnatal stage. We here found that SelW protein significantly increased in mouse brains of postnatal day 8 and 20 relative to embryonic day 15. This was accompanied by increased expression of SOD1 and SOD2. When the expression of SelW in primary cultured cells derived from embryonic cerebral cortex was knocked down with small interfering RNAs (siRNAs), SelW siRNA-transfected neuronal cells were more sensitive to the oxidative stress induced by treatment of H2O2 than control cells. TUNEL assays revealed that H2O2-induced apoptotic cell death occurred at a higher frequency in the siRNA-transfected cells than in the control cells. Taken together, our findings suggest that SelW plays an important role in protection of neurons from oxidative stress during neuronal development.

Keywords: antioxidant, neuronal cells, oxidative stress, selenium, selenoprotein W

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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