Mol. Cells 2009; 27(5): 601-608
Published online May 31, 2009
https://doi.org/10.1007/s10059-009-0080-5
© The Korean Society for Molecular and Cellular Biology
The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK, and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans SK-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 [M + H]+, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of me-tabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of 324 [M + H]+. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.
Keywords aminoglycosides, heterologous expression, kanamycin, paromamine, Streptomyces kanamyceticus
Mol. Cells 2009; 27(5): 601-608
Published online May 31, 2009 https://doi.org/10.1007/s10059-009-0080-5
Copyright © The Korean Society for Molecular and Cellular Biology.
Keshav Kumar Nepal, Tae-Jin Oh, and Jae Kyung Sohng
The 2-deoxystreptamine and paromamine are two key intermediates in kanamycin biosynthesis. In the present study, pSK-2 and pSK-7 recombinant plasmids were constructed with two combinations of genes: kanABK, and kanABKF and kacA respectively from kanamycin producer Streptomyces kanamyceticus ATCC12853. These plasmids were heterologously expressed into Streptomyces lividans TK24 independently and generated two recombinant strains named S. lividans SK-2/SL and S. lividans SK-7/SL, respectively. ESI/ MS and ESI-LC/MS analysis of the metabolite from S. lividans SK-2/SL showed that the compound had a molecular mass of 163 [M + H]+, which corresponds to that of 2-deoxystreptamine. ESI/MS and MS/MS analysis of me-tabolites from S. lividans SK-7/SL demonstrated the production of paromamine with a molecular mass of 324 [M + H]+. In this study, we report the production of paromamine in a heterologous host for the first time. This study will evoke to explore complete biosynthetic pathways of kanamycin and related aminoglycoside antibiotics.
Keywords: aminoglycosides, heterologous expression, kanamycin, paromamine, Streptomyces kanamyceticus
Kum-Kang So, Yun-Jo Chung, Jung-Mi Kim, Beom-Tae Kim, Seung-Moon Park, and Dae-Hyuk Kim
Mol. Cells 2015; 38(12): 1105-1110 https://doi.org/10.14348/molcells.2015.0208Gopal Prasad Ghimire, Tae-Jin Oh, Kwangkyoung Liou and Jae Kyung Sohng
Mol. Cells 2008; 26(4): 362-367 https://doi.org/10.14348/.2008.26.4.362