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Mol. Cells 2009; 27(5): 571-575

Published online May 31, 2009

https://doi.org/10.1007/s10059-009-0075-2

© The Korean Society for Molecular and Cellular Biology

ATG5 Expression Induced by MDMA (Ecstasy), Interferes with Neuronal Differentiation of Neuroblastoma Cells

Myounghee Chae, Gyu-Seek Rhee, Ik-Soon Jang, Kwangsoo Kim, Ji-Hae Lee, Seung-Yeul Lee, Minjung Kim, Junyoung Yang, Junsoo Park, and Seung-Hoon Lee

Received: February 2, 2009; Revised: March 13, 2009; Accepted: March 23, 2009

Abstract

The amphetamine derivative 3, 4-methylenedioxymetham- phetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblas-toma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

Keywords Atg5, autophagy, MDMA, neuron, PC12, SH-SY5Y

Article

Research Article

Mol. Cells 2009; 27(5): 571-575

Published online May 31, 2009 https://doi.org/10.1007/s10059-009-0075-2

Copyright © The Korean Society for Molecular and Cellular Biology.

ATG5 Expression Induced by MDMA (Ecstasy), Interferes with Neuronal Differentiation of Neuroblastoma Cells

Myounghee Chae, Gyu-Seek Rhee, Ik-Soon Jang, Kwangsoo Kim, Ji-Hae Lee, Seung-Yeul Lee, Minjung Kim, Junyoung Yang, Junsoo Park, and Seung-Hoon Lee

Received: February 2, 2009; Revised: March 13, 2009; Accepted: March 23, 2009

Abstract

The amphetamine derivative 3, 4-methylenedioxymetham- phetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblas-toma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

Keywords: Atg5, autophagy, MDMA, neuron, PC12, SH-SY5Y

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

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