Mol. Cells 2009; 27(5): 571-575
Published online May 15, 2009
https://doi.org/10.1007/s10059-009-0075-2
© The Korean Society for Molecular and Cellular Biology
The amphetamine derivative 3, 4-methylenedioxymetham- phetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblas-toma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.
Keywords Atg5, autophagy, MDMA, neuron, PC12, SH-SY5Y
Mol. Cells 2009; 27(5): 571-575
Published online May 31, 2009 https://doi.org/10.1007/s10059-009-0075-2
Copyright © The Korean Society for Molecular and Cellular Biology.
Myounghee Chae, Gyu-Seek Rhee, Ik-Soon Jang, Kwangsoo Kim, Ji-Hae Lee, Seung-Yeul Lee, Minjung Kim, Junyoung Yang, Junsoo Park, and Seung-Hoon Lee
The amphetamine derivative 3, 4-methylenedioxymetham- phetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblas-toma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.
Keywords: Atg5, autophagy, MDMA, neuron, PC12, SH-SY5Y
Jae Hyeong Lee, Sang-Ah Park, Il-Geun Park, Bo Kyung Yoon, Jung-Shin Lee, and Ji Min Lee
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