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Mol. Cells 2009; 27(4): 481-490

Published online April 13, 2009

https://doi.org/10.1007/s10059-009-0054-7

© The Korean Society for Molecular and Cellular Biology

Temporal and Spatial Expression Patterns of Nine Arabidopsis Genes Encoding Jumonji C-Domain Proteins

Eun-Hye Hong, Young-Min Jeong, Jee-Youn Ryu, Richard M. Amasino, Bosl Noh, and Yoo-Sun Noh

Received: February 2, 2009; Accepted: February 10, 2009

Abstract

Diverse posttranslational modifications of histones, such as acetylation and methylation, play important roles in controlling gene expression. Histone methylation in par-ticular is involved in a broad range of biological processes, including heterochromatin formation, X-chromosome inactivation, genomic imprinting, and tran-scriptional regulation. Recently, it has been demonstrated that proteins containing the Jumonji (Jmj) C domain can demethylate histones. In Arabidopsis, twenty-one genes encode JmjC domain-containing proteins, which can be clustered into five clades. To address the biological roles of the Arabidopsis genes encoding JmjC-domain proteins, we analyzed the temporal and spatial expression patterns of nine genes. RT-PCR analyses indicate all nine Arabidopsis thaliana Jmj (AtJmj) genes studied are actively expressed in vari-ous tissues. Furthermore, studies of transgenic plants harboring AtJmj::β-glucuronidase fusion constructs reveal that these nine AtJmj genes are expressed in a developmentally and spatially regulated manner.

Keywords Arabidopsis, demethylase, epigenetics, histone demethylation, Jumonji

Article

Research Article

Mol. Cells 2009; 27(4): 481-490

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0054-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Temporal and Spatial Expression Patterns of Nine Arabidopsis Genes Encoding Jumonji C-Domain Proteins

Eun-Hye Hong, Young-Min Jeong, Jee-Youn Ryu, Richard M. Amasino, Bosl Noh, and Yoo-Sun Noh

Received: February 2, 2009; Accepted: February 10, 2009

Abstract

Diverse posttranslational modifications of histones, such as acetylation and methylation, play important roles in controlling gene expression. Histone methylation in par-ticular is involved in a broad range of biological processes, including heterochromatin formation, X-chromosome inactivation, genomic imprinting, and tran-scriptional regulation. Recently, it has been demonstrated that proteins containing the Jumonji (Jmj) C domain can demethylate histones. In Arabidopsis, twenty-one genes encode JmjC domain-containing proteins, which can be clustered into five clades. To address the biological roles of the Arabidopsis genes encoding JmjC-domain proteins, we analyzed the temporal and spatial expression patterns of nine genes. RT-PCR analyses indicate all nine Arabidopsis thaliana Jmj (AtJmj) genes studied are actively expressed in vari-ous tissues. Furthermore, studies of transgenic plants harboring AtJmj::β-glucuronidase fusion constructs reveal that these nine AtJmj genes are expressed in a developmentally and spatially regulated manner.

Keywords: Arabidopsis, demethylase, epigenetics, histone demethylation, Jumonji

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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