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Mol. Cells 2009; 27(4): 481-490

Published online April 30, 2009

https://doi.org/10.1007/s10059-009-0054-7

© The Korean Society for Molecular and Cellular Biology

Temporal and Spatial Expression Patterns of Nine Arabidopsis Genes Encoding Jumonji C-Domain Proteins

Eun-Hye Hong, Young-Min Jeong, Jee-Youn Ryu, Richard M. Amasino, Bosl Noh, and Yoo-Sun Noh

Received: February 2, 2009; Accepted: February 10, 2009

Abstract

Diverse posttranslational modifications of histones, such as acetylation and methylation, play important roles in controlling gene expression. Histone methylation in par-ticular is involved in a broad range of biological processes, including heterochromatin formation, X-chromosome inactivation, genomic imprinting, and tran-scriptional regulation. Recently, it has been demonstrated that proteins containing the Jumonji (Jmj) C domain can demethylate histones. In Arabidopsis, twenty-one genes encode JmjC domain-containing proteins, which can be clustered into five clades. To address the biological roles of the Arabidopsis genes encoding JmjC-domain proteins, we analyzed the temporal and spatial expression patterns of nine genes. RT-PCR analyses indicate all nine Arabidopsis thaliana Jmj (AtJmj) genes studied are actively expressed in vari-ous tissues. Furthermore, studies of transgenic plants harboring AtJmj::β-glucuronidase fusion constructs reveal that these nine AtJmj genes are expressed in a developmentally and spatially regulated manner.

Keywords Arabidopsis, demethylase, epigenetics, histone demethylation, Jumonji

Article

Research Article

Mol. Cells 2009; 27(4): 481-490

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0054-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Temporal and Spatial Expression Patterns of Nine Arabidopsis Genes Encoding Jumonji C-Domain Proteins

Eun-Hye Hong, Young-Min Jeong, Jee-Youn Ryu, Richard M. Amasino, Bosl Noh, and Yoo-Sun Noh

Received: February 2, 2009; Accepted: February 10, 2009

Abstract

Diverse posttranslational modifications of histones, such as acetylation and methylation, play important roles in controlling gene expression. Histone methylation in par-ticular is involved in a broad range of biological processes, including heterochromatin formation, X-chromosome inactivation, genomic imprinting, and tran-scriptional regulation. Recently, it has been demonstrated that proteins containing the Jumonji (Jmj) C domain can demethylate histones. In Arabidopsis, twenty-one genes encode JmjC domain-containing proteins, which can be clustered into five clades. To address the biological roles of the Arabidopsis genes encoding JmjC-domain proteins, we analyzed the temporal and spatial expression patterns of nine genes. RT-PCR analyses indicate all nine Arabidopsis thaliana Jmj (AtJmj) genes studied are actively expressed in vari-ous tissues. Furthermore, studies of transgenic plants harboring AtJmj::β-glucuronidase fusion constructs reveal that these nine AtJmj genes are expressed in a developmentally and spatially regulated manner.

Keywords: Arabidopsis, demethylase, epigenetics, histone demethylation, Jumonji

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

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