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Mol. Cells 2009; 27(4): 475-480

Published online April 13, 2009

https://doi.org/10.1007/s10059-009-0063-6

© The Korean Society for Molecular and Cellular Biology

Functional Analysis of the Stress-Inducible Soybean Calmodulin Isoform-4 (GmCaM-4) Promoter in Transgenic Tobacco Plants

Hyeong Cheol Park, Man Lyang Kim, Yun Hwan Kang, Jae Cheol Jeong, Mi Sun Cheong, Wonkyun Choi, Sang Yeol Lee, Moo Je Cho, Min Chul Kim, Woo Sik Chung, and Dae-Jin Yun

Received: January 22, 2009; Revised: February 24, 2009; Accepted: February 26, 2009

Abstract

The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expres-sion systems.

Keywords calmodulin, pathogen, promoter, salt stress, soybean (Glycine max), transcription factor

Article

Research Article

Mol. Cells 2009; 27(4): 475-480

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0063-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Functional Analysis of the Stress-Inducible Soybean Calmodulin Isoform-4 (GmCaM-4) Promoter in Transgenic Tobacco Plants

Hyeong Cheol Park, Man Lyang Kim, Yun Hwan Kang, Jae Cheol Jeong, Mi Sun Cheong, Wonkyun Choi, Sang Yeol Lee, Moo Je Cho, Min Chul Kim, Woo Sik Chung, and Dae-Jin Yun

Received: January 22, 2009; Revised: February 24, 2009; Accepted: February 26, 2009

Abstract

The transcription of soybean (Glycine max) calmodulin isoform-4 (GmCaM-4) is dramatically induced within 0.5 h of exposure to pathogen or NaCl. Core cis-acting elements that regulate the expression of the GmCaM-4 gene in response to pathogen and salt stress were previously identified, between -1,207 and -1,128 bp, and between -858 and -728 bp, in the GmCaM-4 promoter. Here, we characterized the properties of the DNA-binding complexes that form at the two core cis-acting elements of the GmCaM-4 promoter in pathogen-treated nuclear extracts. We generated GUS reporter constructs harboring various deletions of approximately 1.3-kb GmCaM-4 promoter, and analyzed GUS expression in tobacco plants transformed with these constructs. The GUS expression analysis suggested that the two previously identified core regions are involved in inducing GmCaM-4 expression in the heterologous system. Finally, a transient expression assay of Arabidopsis protoplasts showed that the GmCaM-4 promoter produced greater levels of GUS activity than did the CaMV35S promoter after pathogen or NaCl treatments, suggesting that the GmCaM-4 promoter may be useful in the production of conditional gene expres-sion systems.

Keywords: calmodulin, pathogen, promoter, salt stress, soybean (Glycine max), transcription factor

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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