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Mol. Cells 2009; 27(4): 467-473

Published online April 13, 2009

https://doi.org/10.1007/s10059-009-0062-7

© The Korean Society for Molecular and Cellular Biology

Identification and Characterization of Alternative Promoters of the Rice MAP Kinase Gene OsBWMK1

Sung Cheol Koo, Man Soo Choi, Hyun Jin Chun, Hyeong Cheol Park, Chang Ho Kang, Sang In Shim, Jong Il Chung, Yong Hwa Cheong, Sang Yeol Lee, Dae-Jin Yun, Woo Sik Chung, Moo Je Cho, and Min Chul Kim

Received: January 19, 2009; Revised: February 24, 2009; Accepted: February 26, 2009

Abstract

Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expres-sion patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.

Keywords alternative promoter, gene expression regulation, MAP kinase, rice, splice variant

Article

Research Article

Mol. Cells 2009; 27(4): 467-473

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0062-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification and Characterization of Alternative Promoters of the Rice MAP Kinase Gene OsBWMK1

Sung Cheol Koo, Man Soo Choi, Hyun Jin Chun, Hyeong Cheol Park, Chang Ho Kang, Sang In Shim, Jong Il Chung, Yong Hwa Cheong, Sang Yeol Lee, Dae-Jin Yun, Woo Sik Chung, Moo Je Cho, and Min Chul Kim

Received: January 19, 2009; Revised: February 24, 2009; Accepted: February 26, 2009

Abstract

Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expres-sion patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.

Keywords: alternative promoter, gene expression regulation, MAP kinase, rice, splice variant

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

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