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Mol. Cells 2009; 27(4): 449-458

Published online April 13, 2009

https://doi.org/10.1007/s10059-009-0055-6

© The Korean Society for Molecular and Cellular Biology

Ectopic Expression of a Cold-Responsive OsAsr1 cDNA Gives Enhanced Cold Tolerance in Transgenic Rice Plants

Soo-Jin Kim, Sang-Choon Lee, Soon Kwan Hong, Kyungsook An, Gynheung An, and Seong-Ryong Kim

Received: December 31, 2009; Revised: February 9, 2009; Accepted: February 16, 2009

Abstract

The OsAsr1 cDNA clone was isolated from a cDNA library prepared from developing seed coats of rice (Oryza sativa L.). Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs. In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma. For transgenic rice plants that over-expressed full-length OsAsr1 cDNA in the sense orientation, the Fv/Fm values for photosynthetic efficiency were about 2-fold higher than those of wild type-segregating plants after a 24-h cold treatment. Seedlings exposed to prolonged low temperatures were more tolerant of cold stress, as demonstrated during wilting and regrowth tests. Interestingly, OsAsr1 was highly expressed in transgenic rice plants expressing the C-repeat/dehyhdration respon-sive element binding factor 1 (CBF1), suggesting the regulation of OsAsr1 by CBF1. Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance.

Keywords Asr gene, cold tolerance, rice, transgenic plants

Article

Research Article

Mol. Cells 2009; 27(4): 449-458

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0055-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Ectopic Expression of a Cold-Responsive OsAsr1 cDNA Gives Enhanced Cold Tolerance in Transgenic Rice Plants

Soo-Jin Kim, Sang-Choon Lee, Soon Kwan Hong, Kyungsook An, Gynheung An, and Seong-Ryong Kim

Received: December 31, 2009; Revised: February 9, 2009; Accepted: February 16, 2009

Abstract

The OsAsr1 cDNA clone was isolated from a cDNA library prepared from developing seed coats of rice (Oryza sativa L.). Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs. In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma. For transgenic rice plants that over-expressed full-length OsAsr1 cDNA in the sense orientation, the Fv/Fm values for photosynthetic efficiency were about 2-fold higher than those of wild type-segregating plants after a 24-h cold treatment. Seedlings exposed to prolonged low temperatures were more tolerant of cold stress, as demonstrated during wilting and regrowth tests. Interestingly, OsAsr1 was highly expressed in transgenic rice plants expressing the C-repeat/dehyhdration respon-sive element binding factor 1 (CBF1), suggesting the regulation of OsAsr1 by CBF1. Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance.

Keywords: Asr gene, cold tolerance, rice, transgenic plants

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

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