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Mol. Cells 2009; 27(4): 443-447

Published online April 13, 2009

https://doi.org/10.1007/s10059-009-0057-4

© The Korean Society for Molecular and Cellular Biology

Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.

Vladan Ondrej, Miloslav Kitner, Ivana Dolezalova, Petr Nadvornik, Bozena Navratilova, and Ales Lebeda

Received: December 30, 2009; Revised: February 16, 2009; Accepted: February 17, 2009

Abstract

This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the re-entry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a higher variability in the expression profile than did leaves’ mesophyll cells and protoplasts.

Keywords cell cycle, chromatin, plant protoplasts, subtelomeric repeats

Article

Research Article

Mol. Cells 2009; 27(4): 443-447

Published online April 30, 2009 https://doi.org/10.1007/s10059-009-0057-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.

Vladan Ondrej, Miloslav Kitner, Ivana Dolezalova, Petr Nadvornik, Bozena Navratilova, and Ales Lebeda

Received: December 30, 2009; Revised: February 16, 2009; Accepted: February 17, 2009

Abstract

This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the re-entry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a higher variability in the expression profile than did leaves’ mesophyll cells and protoplasts.

Keywords: cell cycle, chromatin, plant protoplasts, subtelomeric repeats

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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