Mol. Cells 2009; 27(3): 321-327
Published online March 19, 2009
https://doi.org/10.1007/s10059-009-0041-z
© The Korean Society for Molecular and Cellular Biology
Voltage-dependent anion channels (VDACs) are reported to be porin-type, ?-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol. In this study, we have investigated a family of VDAC isoforms in Arabidopsis thaliana (AtVDAC). We have shown that the heterologous expression of AtVDAC proteins can functionally complement a yeast mutant lacking the endogenous mitochondrial VDAC gene. AtVDACs tagged with GFP were localized to mitochondria in both yeast and plant cells. We also looked at the response of AtVDACs to biotic and abiotic stresses and found that four AtVDAC transcripts were rapidly up-regulated in response to a bacterial pathogen.
Keywords Arabidopsis, mitochondria, outer membrane, pathogen, voltage-dependent anion channel
Mol. Cells 2009; 27(3): 321-327
Published online March 31, 2009 https://doi.org/10.1007/s10059-009-0041-z
Copyright © The Korean Society for Molecular and Cellular Biology.
Sang Min Lee, My Hanh Thi Hoang, Hay Ju Han, Ho Soo Kim, Kyunghee Lee, Kyung Eun Kim, Doh Hoon Kim, Sang Yeol Lee, and Woo Sik Chung
Voltage-dependent anion channels (VDACs) are reported to be porin-type, ?-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol. In this study, we have investigated a family of VDAC isoforms in Arabidopsis thaliana (AtVDAC). We have shown that the heterologous expression of AtVDAC proteins can functionally complement a yeast mutant lacking the endogenous mitochondrial VDAC gene. AtVDACs tagged with GFP were localized to mitochondria in both yeast and plant cells. We also looked at the response of AtVDACs to biotic and abiotic stresses and found that four AtVDAC transcripts were rapidly up-regulated in response to a bacterial pathogen.
Keywords: Arabidopsis, mitochondria, outer membrane, pathogen, voltage-dependent anion channel
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