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Mol. Cells 2009; 27(2): 199-203

Published online February 28, 2009

https://doi.org/10.1007/s10059-009-0024-0

© The Korean Society for Molecular and Cellular Biology

Ankrd7, a Novel Gene Specifically Expressed in Sertoli Cells and Its Potential Roles in Sertoli Cell Maturation

Yu-Qiang Shi, Lian-Cai Du, Qing-Zhong Wang, Chun-Fang Han

Received: October 23, 2008; Revised: November 18, 2008; Accepted: November 27, 2008

Abstract

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a matu-ration-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermato-genesis.

Keywords Ankrd7, Ankyrin repeat motif, maturation, Sertoli cells

Article

Research Article

Mol. Cells 2009; 27(2): 199-203

Published online February 28, 2009 https://doi.org/10.1007/s10059-009-0024-0

Copyright © The Korean Society for Molecular and Cellular Biology.

Ankrd7, a Novel Gene Specifically Expressed in Sertoli Cells and Its Potential Roles in Sertoli Cell Maturation

Yu-Qiang Shi, Lian-Cai Du, Qing-Zhong Wang, Chun-Fang Han

Received: October 23, 2008; Revised: November 18, 2008; Accepted: November 27, 2008

Abstract

The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a matu-ration-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immuno-histochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermato-genesis.

Keywords: Ankrd7, Ankyrin repeat motif, maturation, Sertoli cells

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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