Mol. Cells 2001; 12(1): 17-24
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
We investigated the role of wild-type (wt)-p53 as an inducer of apoptotic cell death in human hepatoma cell lines. Following the retrovirus-mediated transduction of the wt-p53 gene, Hep3B cells lacking the endogenous p53 expression began to die through apoptosis in 4 h. They showed a maximal apoptotic death at 12 h, whereas HepG2 cells expressing endogenous p53 did not. However, the transduction of the wt-p53 gene elicited growth suppression of both Hep3B and HepG2 cells. P21WAF1/CIP1, a p53-inducible cell cycle inhibitor, was induced, not only in Hep3B cells undergoing apoptosis, but also in HepG2 cells. The kinetics of the p21WAF1/CIP1 induction, DNA fragmentation, and growth suppression of the Hep3B cells showed that DNA fragmentation and growth suppression progressed rapidly following p21WAF1/CIP1 accumulation. N-acetyl-cysteine or glutathione, potent antioxidants, strongly inhibited the DNA fragmentation, but did not reduce the elevated level of p21WAF1/CIP1. These findings suggested that p21WAF1/CIP1 was not a critical mediator for the execution of p53-mediated apoptosis, although it contributed to the growth inhibition of cells undergoing apoptosis. Furthermore, p53-mediated apoptosis could be repressed by antioxidants.
Keywords Apoptosis, p53, : p21WAF1/CIP1, Hepat, Antioxidant
Mol. Cells 2001; 12(1): 17-24
Published online August 31, 2001
Copyright © The Korean Society for Molecular and Cellular Biology.
Kee-Ho Lee, Keun-Cheol Kim, Yu-Jin Jung, Yong-Ho Ham, Ja-June Jang, Heechung Kwon
We investigated the role of wild-type (wt)-p53 as an inducer of apoptotic cell death in human hepatoma cell lines. Following the retrovirus-mediated transduction of the wt-p53 gene, Hep3B cells lacking the endogenous p53 expression began to die through apoptosis in 4 h. They showed a maximal apoptotic death at 12 h, whereas HepG2 cells expressing endogenous p53 did not. However, the transduction of the wt-p53 gene elicited growth suppression of both Hep3B and HepG2 cells. P21WAF1/CIP1, a p53-inducible cell cycle inhibitor, was induced, not only in Hep3B cells undergoing apoptosis, but also in HepG2 cells. The kinetics of the p21WAF1/CIP1 induction, DNA fragmentation, and growth suppression of the Hep3B cells showed that DNA fragmentation and growth suppression progressed rapidly following p21WAF1/CIP1 accumulation. N-acetyl-cysteine or glutathione, potent antioxidants, strongly inhibited the DNA fragmentation, but did not reduce the elevated level of p21WAF1/CIP1. These findings suggested that p21WAF1/CIP1 was not a critical mediator for the execution of p53-mediated apoptosis, although it contributed to the growth inhibition of cells undergoing apoptosis. Furthermore, p53-mediated apoptosis could be repressed by antioxidants.
Keywords: Apoptosis, p53, : p21WAF1/CIP1, Hepat, Antioxidant
Youngleem Kim, Dai-Wu Seol
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