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Mol. Cells 2001; 12(3): 368-371

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

The Expression of Bok Is Regulated by Serum in HC11 Mammary Epithelial Cells

Seck-Ho Ha, Sul-Ra Lee, Tae-Hoon Lee, Young-Min Kim, Myung-Gi Baik, Yun-Jaie Choi

Abstract

Epithelial cells within the mammary gland undergo apoptosis during weaning. To determine the expression of Bok mRNA (a member of the pro-apoptotic Bcl-2 family) in the mammary gland and its regulation, we examined the expression of the Bok transcript in the mouse mammary gland and HC11 mammary epithe-lial cells in culture through RT-PCR. The Bok mRNA expression was found in the mammary gland. The ex-pression of the Bok mRNA level was induced through serum starvation and overexpression of Bok induced apoptosis in HC11 cells in culture. These results indi-cate that the expression of Bok mRNA in the mam-mary gland is regulated through serum starvation. It also may be related to the mammary involution.

Keywords Bok, Ser, Apoptosis, HC11 Mammary Epithelial Cells

Article

Research Article

Mol. Cells 2001; 12(3): 368-371

Published online December 31, 2001

Copyright © The Korean Society for Molecular and Cellular Biology.

The Expression of Bok Is Regulated by Serum in HC11 Mammary Epithelial Cells

Seck-Ho Ha, Sul-Ra Lee, Tae-Hoon Lee, Young-Min Kim, Myung-Gi Baik, Yun-Jaie Choi

Abstract

Epithelial cells within the mammary gland undergo apoptosis during weaning. To determine the expression of Bok mRNA (a member of the pro-apoptotic Bcl-2 family) in the mammary gland and its regulation, we examined the expression of the Bok transcript in the mouse mammary gland and HC11 mammary epithe-lial cells in culture through RT-PCR. The Bok mRNA expression was found in the mammary gland. The ex-pression of the Bok mRNA level was induced through serum starvation and overexpression of Bok induced apoptosis in HC11 cells in culture. These results indi-cate that the expression of Bok mRNA in the mam-mary gland is regulated through serum starvation. It also may be related to the mammary involution.

Keywords: Bok, Ser, Apoptosis, HC11 Mammary Epithelial Cells

Mol. Cells
Jun 30, 2023 Vol.46 No.6, pp. 329~398
COVER PICTURE
The cellular proteostasis network is adaptively modulated upon cellular stress, thereby protecting cells from proteostasis collapse. Heat shock induces the translocation of misfolded proteins and the chaperone protein HSP70 into nucleolus, where nuclear protein quality control primarily occurs. Nuclear RNA export factor 1 (green), nucleolar protein fibrillarin (red), and nuclei (blue) were visualized in NIH3T3 cells under basal (left) and heat shock (right) conditions (Park et al., pp. 374-386).

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