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Mol. Cells 2001; 12(3): 368-371

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

The Expression of Bok Is Regulated by Serum in HC11 Mammary Epithelial Cells

Seck-Ho Ha, Sul-Ra Lee, Tae-Hoon Lee, Young-Min Kim, Myung-Gi Baik, Yun-Jaie Choi

Abstract

Epithelial cells within the mammary gland undergo apoptosis during weaning. To determine the expression of Bok mRNA (a member of the pro-apoptotic Bcl-2 family) in the mammary gland and its regulation, we examined the expression of the Bok transcript in the mouse mammary gland and HC11 mammary epithe-lial cells in culture through RT-PCR. The Bok mRNA expression was found in the mammary gland. The ex-pression of the Bok mRNA level was induced through serum starvation and overexpression of Bok induced apoptosis in HC11 cells in culture. These results indi-cate that the expression of Bok mRNA in the mam-mary gland is regulated through serum starvation. It also may be related to the mammary involution.

Keywords Bok, Ser, Apoptosis, HC11 Mammary Epithelial Cells

Article

Research Article

Mol. Cells 2001; 12(3): 368-371

Published online December 31, 2001

Copyright © The Korean Society for Molecular and Cellular Biology.

The Expression of Bok Is Regulated by Serum in HC11 Mammary Epithelial Cells

Seck-Ho Ha, Sul-Ra Lee, Tae-Hoon Lee, Young-Min Kim, Myung-Gi Baik, Yun-Jaie Choi

Abstract

Epithelial cells within the mammary gland undergo apoptosis during weaning. To determine the expression of Bok mRNA (a member of the pro-apoptotic Bcl-2 family) in the mammary gland and its regulation, we examined the expression of the Bok transcript in the mouse mammary gland and HC11 mammary epithe-lial cells in culture through RT-PCR. The Bok mRNA expression was found in the mammary gland. The ex-pression of the Bok mRNA level was induced through serum starvation and overexpression of Bok induced apoptosis in HC11 cells in culture. These results indi-cate that the expression of Bok mRNA in the mam-mary gland is regulated through serum starvation. It also may be related to the mammary involution.

Keywords: Bok, Ser, Apoptosis, HC11 Mammary Epithelial Cells

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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