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Mol. Cells

Published online July 28, 2022

© The Korean Society for Molecular and Cellular Biology

The Peripheral Immune Landscape in a Patient with Myocarditis after the Administration of BNT162b2 mRNA Vaccine

Bo Kyung Yoon1,2,10 , Tae Gyu Oh3,10 , Seonghyeon Bu4,5,10 , Kyung Jin Seo6 , Se Hwan Kwon7 , Ji Yoon Lee8 , Yeumin Kim8 , Jae-woo Kim1,2 , Hyo-Suk Ahn4,5,* , and Sungsoon Fang2,8,9,*

1Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul 03722, Korea, 2Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, Korea, 3Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA, 4Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Uijeongbu St. Mary’s Hospital, Seoul 06591, Korea, 5Catholic Research Institute for Intractable Cardiovascular Disease (CRID), College of Medicine, The Catholic University of Korea, Seoul 06591, Korea, 6Department of Pathology, The Catholic University of Korea, Uijeongbu St. Mary’s Hospital, Seoul 06591, Korea, 7Department of Radiology, Kyung Hee University Medical Center, Seoul 02447, Korea, 8KYNOGEN Co., Suwon 16229, Korea, 9Severance Biomedical Science Institute, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Korea, 10These authors contributed equally to this work.

Correspondence to : alaco0502@gmail.com (HSA); sfang@yuhs.ac (SF)

Received: February 24, 2022; Revised: May 27, 2022; Accepted: June 9, 2022

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed a serious threat to global public health. A novel vaccine made from messenger RNA (mRNA) has been developed and approved for use at an unprecedented pace. However, an increased risk of myocarditis has been reported after BNT162b2 mRNA vaccination due to unknown causes. In this study, we used single-cell RNA sequencing and single-cell T cell receptor sequencing analyses of peripheral blood mononuclear cells (PBMCs) to describe, for the first time, changes in the peripheral immune landscape of a patient who underwent myocarditis after BNT162b2 vaccination. The greatest changes were observed in the transcriptomic profile of monocytes in terms of the number of differentially expressed genes. When compared to the transcriptome of PBMCs from vaccinated individuals without complications, increased expression levels of IL7R were detected in multiple cell clusters. Overall, results from this study can help advance research into the pathogenesis of BNT162b2-induced myocarditis.

Keywords BNT162b2, myocarditis, peripheral blood mononucleear cells, severe acute respiratory syndrome coronavirus 2, single-cell RNA sequencing

Article

On-line First

Mol. Cells

Published online July 28, 2022

Copyright © The Korean Society for Molecular and Cellular Biology.

The Peripheral Immune Landscape in a Patient with Myocarditis after the Administration of BNT162b2 mRNA Vaccine

Bo Kyung Yoon1,2,10 , Tae Gyu Oh3,10 , Seonghyeon Bu4,5,10 , Kyung Jin Seo6 , Se Hwan Kwon7 , Ji Yoon Lee8 , Yeumin Kim8 , Jae-woo Kim1,2 , Hyo-Suk Ahn4,5,* , and Sungsoon Fang2,8,9,*

1Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul 03722, Korea, 2Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, Korea, 3Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA, 4Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Uijeongbu St. Mary’s Hospital, Seoul 06591, Korea, 5Catholic Research Institute for Intractable Cardiovascular Disease (CRID), College of Medicine, The Catholic University of Korea, Seoul 06591, Korea, 6Department of Pathology, The Catholic University of Korea, Uijeongbu St. Mary’s Hospital, Seoul 06591, Korea, 7Department of Radiology, Kyung Hee University Medical Center, Seoul 02447, Korea, 8KYNOGEN Co., Suwon 16229, Korea, 9Severance Biomedical Science Institute, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Korea, 10These authors contributed equally to this work.

Correspondence to:alaco0502@gmail.com (HSA); sfang@yuhs.ac (SF)

Received: February 24, 2022; Revised: May 27, 2022; Accepted: June 9, 2022

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed a serious threat to global public health. A novel vaccine made from messenger RNA (mRNA) has been developed and approved for use at an unprecedented pace. However, an increased risk of myocarditis has been reported after BNT162b2 mRNA vaccination due to unknown causes. In this study, we used single-cell RNA sequencing and single-cell T cell receptor sequencing analyses of peripheral blood mononuclear cells (PBMCs) to describe, for the first time, changes in the peripheral immune landscape of a patient who underwent myocarditis after BNT162b2 vaccination. The greatest changes were observed in the transcriptomic profile of monocytes in terms of the number of differentially expressed genes. When compared to the transcriptome of PBMCs from vaccinated individuals without complications, increased expression levels of IL7R were detected in multiple cell clusters. Overall, results from this study can help advance research into the pathogenesis of BNT162b2-induced myocarditis.

Keywords: BNT162b2, myocarditis, peripheral blood mononucleear cells, severe acute respiratory syndrome coronavirus 2, single-cell RNA sequencing

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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