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Fig. 4. Endogenous SYT5 is localized to the perinuclear region in Arabidopsis roots. (A-C) Cellular localization of endogenous SYT5 in roots of transgenic plants expressing the PM-localized GFP-SYP132. Endogenous SYT5 was detected by anti-SYT5 antibody and Alexa 546 (red fluorescence)-conjugated anti-rabbit IgG antibody. Fluoresent images were analyzed by confocal microscopy. Scale bars = 10 μm. (D-F) Cellular localization of endogenous SYT5 in roots of transgenic plants expressing the TGN/secretory vesicle-localized mRFP-VAMP722. Endogenous SYT5 was detected by anti-SYT5 antibody and Alexa 488 (green fluorescence)-conjugated anti-rabbit IgG antibody. Fluoresent images were analyzed by confocal microscopy. Scale bars = 10 μm.
Mol. Cells 2021;44:670~679 https://doi.org/10.14348/molcells.2021.0100
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