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Fig. 1. SYT5 is required for defense against Pst DC3000 bacterium but not for E. pisi fungus. (A) A schematic view of SYT5 gene. Two independent T-DNA insertion sites for syt5-1 (SALK_036961) and syt5-2 (GABI_679H10) were marked by triangles. LB, left boarder of inserted T-DNA. Box, exon; line, intron. Homozygosity of syt5-1 and syt5-2 plants were tested by immunoblot. Proteins extracted from the indicated genotype plants were subject to immunoblot with anti-SYT5 and anti-SYP121 antibodies. Equal loading was visualized by staining Rubisco with Coomassie blue. (B) Dispensability of SYT5 for resistance to E. pisi. The indicated genotype plants were inoculated with E. pisi conidiospores. At 2 dpi, inoculated leaves were stained with Commassie blue and analyzed for fungal entry rates. Conidiospores with secondary epiphytic hyphae were regarded as successfully entered fungi. Values are presented as mean ± SE from three biological replications. (C) Elevated Pst DC3000 growth in syt5 plants. The indicated genotype plants were dip-inoculated with Pst DC3000 (1 × 107 cfu). The in-leaf bacterial growth was measured at 3 hpi (gray) or at 3 dpi (black). Values are presented as mean ± SE from four biological replications. *P < 0.05; **P < 0.01 in comparison to WT (Student t-test).
Mol. Cells 2021;44:670~679
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