Fig. 7. Strategies of bisulfite sequencing (BS-seq) library construction.
Input tissues are degraded by freeze-thaw with proteinase K incubation, or by grinding with a pestle, according to the size and the shape of each sample. If the weight of the sample is under 200 mg, we perform DNA extraction with pre-RNase A treatment. The cetyltrimethylammonium bromide (CTAB) method (Allen et al., 2006
) is generally used for genomic DNA extraction (identified with an asterisk).
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