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Fig. 2. A summary of sampling methods according to developmental stage.
Three steps (release, collection, and washing and confirm) were optimized for each embryo stage. The heart stage embryo was dissected for sampling first horizontally then vertically. The diameters of S shaped glass micropipettes used were 50 μm and 100 μm for globular and heart stage embryos, respectively. The washing step was conducted repeatedly until embryos are purified.
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