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Fig. 3. Effects of TULPs on ciliary trafficking of IFT140 in RPE1 cells.
(A) Wild-type RPE1 cells and TULP3 KO RPE1 cells, transfected with the indicated TULPs. Cells were stained with antibodies specific for the axonemal marker Ac-tubulin and IFT140. Transfected cells were identified with DsRed staining. Non-transfected cells were identified with the nuclear stain To-Pro3. Insets, enlarged images of the cilia marked with arrowheads. Scale bars = 5 μm. (B) Quantification of IFT140 localization. Data are presented as mean ± SEM from three independent experiments. Total counted cells are 100-150 for each condition. Pearson’s χ2 test. ****P < 0.0001.
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