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Fig. 1. Effects of TULPs on ciliogenesis in RPE1 cells.
(A) Wild-type RPE1 cells and TULP3 KO RPE1 cells, transfected with the indicated TULPs. Cells were stained with an antibody specific to the axonemal marker Ac-tubulin. Transfected cells were identified with DsRed staining. Non-transfected cells were identified with the nuclear stain To-Pro3. Scale bars = 5 μm. (B and C) Quantification of ciliated cells (B) and cilia length (C). Data are presented as mean ± SEM from three independent experiments. More than 300 cells were counted for each genotype for the ciliary formation experiment and more than 100 were counted for each genotype for the ciliary length experiment. One-way ANOVAs with Tukey’s post-hoc tests. ***P < 0.001.
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