Fig. 5EThe inhibitory effect of hUC-MSCs on type 2 cytokine production from murine Th2 cells and PBMC from asthmatics.
(A-D) In vitro
differentiated Th1, Th2, and Th17 cells were co-cultured with hUC-MSCs (A), after which FACS was used to measure the IFNγ (B), IL-5, IL-13 (C), and IL-17A (D) produced by the Th1, Th2, and Th17 cells, respectively. (E) PBMCs isolated from asthma patients were co-cultured for 24 h with hUC-MSCs (left panel), after which the mRNA expression of IL4, IL5,
in the PBMCs (right panels) was measured. The data in Figs. 5B
represent 3 independent experiments (n = 3 for each group). The data in
are from a single experiment with PBMC from five asthma patients. All data are expressed as mean ± SEM. Groups were compared by unpaired (B-D) or paired two-tailed Student’s t
-test (E). *P
˂ 0.05; **P
˂ 0.01; n.s., not significant.
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