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Fig. 4. Kcnma1 ablation leads to a decrease in β-catenin.
(A) Representative images of β-catenin staining of tibiae from the indicated groups of mice. Scale bars = 100 μm. (B) Immunofluorescent staining of β-catenin in osteoblasts isolated from the indicated mice. (C) Protein expression of Runx2 and β-catenin in ROS 17/2.8 cells determined by western blot. ROS 17/2.8 cells were treated with different concentrations of paxilline for 48 h. (D and E) Quantitative analysis of Runx2 and β-catenin in vitro. (F) The Runx2 mRNA expression levels in ROS 17/2.8 cells treated with different concentrations of paxilline. (G) Cell viability of ROS 17/2.8 cells was assessed by CCK-8 assay. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. (H) Western blot analysis of Runx2, β-catenin and BK after the BKα-myc plasmid was transfected into MC3T3-E1 cells. (I) Quantitative analysis of Runx2, β-catenin and BK in BKα-myc plasmid transfected cells. Quantification of protein levels was normalized to GAPDH. Data are presented as mean ± SD, versus wild type (WT) –: *P < 0.05, **P < 0.01, ***P < 0.001, versus KO –: #P < 0.05, ##P < 0.01.
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